The intrabody targeting of hTERT attenuates the immortality of cancer cells

• Autorzy: Zhu X , Yang N. , Cai J. , Yang G. , Liang S. , Ren D.
• Języki publikacji: EN

Abstrakty

EN

hTERT (human telomerase reverse transcriptase) plays a key role in the process of cell immortalization. Overexpression of hTERT has been implicated in 85% of malignant tumors and offers a specific target for cancer therapy. In this paper, we describe an effective approach using a single-chain variable fragment (scFv) intrabody derived from monoclonal hybridoma directed against hTERT to attenuate the immortalization of human uterine cervix and hepatoma cells. The scFv we constructed had a high affinity to hTERT, and specifically neutralized over 70% of telomere synthesis activity, thereby inhibiting the viability and proliferation of the cancer cells. Our results indicate that this anti-hTERT intrabody is a promising tool to target hTERT and intervene in the immortalization process of cancer cells.

Słowa kluczowe

EN

intrabody; immortality; cancer cell; telomerase reverse transcriptase; man; cell immortalization; cancer therapy

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2010
• Tom: 15
• Numer: 1
• Opis fizyczny: p.32-45,fig.,ref.

Twórcy

autor

Zhu X

• Fudan University, Fudan, P.R.China

autor

Yang N.

autor

Cai J.

autor

Yang G.

autor

Liang S.

autor

Ren D.

Bibliografia

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