Molecular mechanism of protection against chemically and gamma-radiation induced apoptosis in human colon cancer cells

• Autorzy: Pierzchalski P. , Krawiec A. , Gawelko J. , Pawlik W.W. , Konturek S.J. , Gonciarz M.
• Języki publikacji: EN

Abstrakty

EN

The involvement of peroxisome proliferator-activated receptors (PPARs) in the cancer cell elimination through apoptosis is a generally accepted fact. However, some reports indicate that the activation of PPAR is directly responsible for carcinogenesis. Caco-2 cells, a human adenocarcinoma cells, were used as a model of colon cancer. Cell cultures (5x106 cell per dish) were pretreated for 24h with PPARagonists ciglitazone (CI, 1x10-6M) and retinoic acid (RA, 1x10-6M) and part of the cultures were subsequently subjected to -radiation (photons) with therapeutic dose of 2,5 Gy. Total cellular RNA and proteins (cytoplasmic and nuclear) were isolated 24h after cultures irradiation or 48h after stimulation in the non irradiated part of experiment to preserve the equal growth time for all samples. -Irradiation of the cells abolished nuclear translocation of PPARunder its agonists treatment and preserved PPARin the cytoplasmic pool. But it did not affect the HSP 70 expression in response to ciglitazone and retinoic acid. Moreover, combined -irradiation and CI/RA treatment of the cells changed the equilibrium between Bax and Bcl-2 mRNA to anti apoptotic state with increased expression of Bcl-2 and almost abolished expression of Bax. In conclusion, this paper provides an evidence for the anti-apoptotic action of PPARagonists used along with the -radiation. Moreover, it shows that the up-regulated HSP70, in response to PPARagonists in -irradiated cultures promotes cell survival.

Słowa kluczowe

EN

man; cancer; colon cancer cell; molecular mechanism; apoptosis; embryogenesis; carcinogenesis; gamma-radiation; maturation; immune system; homeostasis; peroxisome proliferator-activated receptor

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2008
• Tom: 59
• Numer: 2
• Opis fizyczny: p.191-202,fig.,ref.

Twórcy

autor

Pierzchalski P.

• Jagiellonian University Medical College, 16 Grzegorzecka Street, 31-531 Krakow, Poland

autor

Krawiec A.

autor

Gawelko J.

autor

Pawlik W.W.

autor

Konturek S.J.

autor

Gonciarz M.