Effect of L-arginine on lead induced oxidative stress in the blood of rats with different resistance to hypoxia

• Autorzy: Tkachenko H. , Kurhalyuk N. , Khabrovska L. , Kminski P.

Warianty tytułu

PL

Wplyw L-argininy na stres oksydacyjny wywolany olowiem we krwi szczurow z rozna odpornoscia na niedotlenienie

• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to estimate beneficial effects of L-arginine, a nitric oxide precursor, on antioxidant enzymes activity (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, ceruloplasmine), the lipid peroxidation processes level and parameters of membrane erythrocytes resistance before and after lead intoxication in rats with different resistance to hypoxia. Our results suggest that the antioxidant system enzymes activity and lipid peroxidation processes level in animals which differ in sensitiveness to hypoxia, are higher in animals with low resistance to hypoxia in the control group. We have shown that the amino acid, L-arginine, is an efficient antioxidant capable of reducing the level of lipid peroxidation processes in blood of lead-preexposed rats. L-arginine treatment under lead intoxication caused alteration in antioxidant enzymes activity due to increasing the enzymes activity of glutathione system, especially in animals with low resistance to hypoxia. The influence of L-arginine under lead intoxication was investigated to ascertain whether this amino acid possesses antioxidant properties before lead injection (preventive effect) and whether L-arginine has therapeutic effects by treatment after lead intoxication. We have shown a significant protective effect of L-arginine under treatment with a preventive effect before lead intoxication. These studies suggest that L-arginine may be a useful drug in treatment under lead intoxication.

PL

Zamierzeniem pracy było określenie korzystnych efektów działania L-argininy, prekursora tlenku azotu, na aktywność enzymów antyoksydacyjnych (dysmutazy ponadtlenkowej, katalazy, glutationreduktazy, glutationperoksydazy, ceruloplazminy), intensywność procesów peroksydacji lipidów i parametry odporności błony erytrocytów, przed i po intoksykacji ołowiem, u szczurów z różną odpornością na niedotlenienie. Nasze wyniki sugerują, że aktywność enzymów antyoksydacyjnych i poziom procesów lipoperoksydacji, u zwierząt różniących się wrażliwością na niedotlenienie, są wyższe u osobników z niską odpornością na niedotlenienie w grupie kontrolnej. Wykazaliśmy, że aminokwas L-arginina jest efektywnym antyoksydantem, zdolnym do zmniejszania intensywności procesów lipoperoksydacji we krwi szczurów eksponowanych na ołów. Traktowanie L-argininą w warunkach intoksykacji ołowiem powoduje zmianę aktywności enzymów antyoksydacyjnych, spowodowanym wzrostem aktywności enzymów systemu glutationu, w szczególności u zwierząt z niską odpornością na niedotlenienie. Wpływ L-argininy w warunkach intoksykacji ołowiem zbadaliśmy w celu określenia, czy aminokwas ten determinuje właściwości antyoksydacyjne przed intoksykacją ołowiem (efekt prewencyjny) oraz, czy L-arginina wykazuje efekty terapeutyczne w wyniku jej oddziaływania po intoksykacji ołowiem. Wykazaliśmy znaczący rezultat ochronny L-argininy w warunkach działania efektu prewencyjnego przed intoksykacją ołowiem. Nasze badania sugerują, że L-arginina może być użytecznym lekiem w warunkach intoksykacji ołowiem.

Słowa kluczowe

EN

hypoxia; rat; blood; oxidative stress; lead; L-arginine; resistance

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 3
• Opis fizyczny: p.387-393,fig.,ref.

Twórcy

autor

Tkachenko H.

• Danylo Halytskiy Lviv National Medical University, Lviv, Ukraine

autor

Kurhalyuk N.

autor

Khabrovska L.

autor

Kminski P.

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