Zastosowanie metody elektroejakulacji do pobrania i oceny nasienia nieplodnego geparda

• Autorzy: Max A , Grabiec A. , Pietrak M. , Balucinska B.

Warianty tytułu

EN

Electroejaculation and semen characteristics in an infertile cheetah

• Języki publikacji: PL

Abstrakty

EN

The cheetah (Acinonyx jubatus) is one of highly endangered species because of low biodiversity, loss of habitat in the wild and reproductive problems in zoos, partially of male origin. The 11-year-old cheetah had no offspring in the past. Semen was collected by electroejaculation. The procedure was performed under general anesthesia using 3.5 mg of xylasine and 5.7 mg of ketamine per kg of body weight. An endorectal electrode was used and electrical stimuli were applied until ejaculation, which occurred as the result of 6 impulses. The 1 ml of semen collected was of olive colour, milky consistency and cat odour. The semen was evaluated microscopically as semi-dense. The progressive movement was observed in 15% of spermatozoa and 50% of spermatozoa were estimated as morphologically normal. The blood testosterone concentration amounted to <0,2 ng/ml. In conclusion, electroejaculation could be an effective method of semen collection in cheetah.

Słowa kluczowe

PL

zwierzeta ogrodow zoologicznych; gepard; nieplodnosc; samce; nasienie; jakosc; ocena jakosci; pobieranie nasienia; metody; elektroejakulacja; morfologia plemnikow; ruch plemnikow; wady plemnikow

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2009
• Tom: 65
• Numer: 05
• Opis fizyczny: s.323-325,fot.,bibliogr.

Twórcy

autor

Max A

• Szkola Glowna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159 C, 02-776 Warszawa

autor

Grabiec A.

autor

Pietrak M.

autor

Balucinska B.

Bibliografia

• 1. Axner E., Holst B. S., Linde-Forsberg C.: Morphology of spermatozoa in the cauda epididymidis before and after electroejaculation and a comparison with ejaculated spermatozoa in the domestic cat. Theriogenology 1998, 50, 973-979.
• 2. Axner E., Ström B., Linde-Forsberg C.: Sperm morphology is better in the second ejaculate than in the first in domestic cats electroejaculated twice during the same period of anesthesia. Theriogenology 1997, 47, 929-934.
• 3. Bertschinger H. J., Jago M., Nöthling J. O., Human A.: Repeated use of the GnRH analogue deslorelin to down-regulate reproduction in male cheetahs (Acinonyx jubatus). Theriogenology 2006, 66, 1762-1767.
• 4. Crosier A. E., Pukazhenthi B. S., Henghali J. N., Howard J., Dickman A. J., Marker L., Wildt D. E.: Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival. Cryobiology 2006, 52, 169-181.
• 5. Donoghue A. M., Howard J. G., Byers A. P., Goodrowe K. L., Bush M., Blumer E., Lukas J., Stover J., Snodgrass K., Wildt D. E.: Correlation of sperm viability with gamete interaction and fertilization in vitro in the cheetah (Acinonyx jubatus). Biol. Reprod. 1992, 46, 1047-1056.
• 6. Dooley M. P., Pineda M. H.: Effect of method of collectionon seminal characteristics of the domestic cat. Am. J. Vet. Res. 1986, 47, 286-292.
• 7. Howard J. G., Roth T. L., Byers A. P., Swanson W. F., Wildt D. E.: Sensitivity to exogenous gonadotropins for ovulation induction and laparoscopic artificial insemination in the cheetah and clouded leopard. Biol. Reprod. 1997, 56, 1059-1068.
• 8. Johnstone I.: Electroejaculation in the domestic cat. Aust. Vet. J. 1984, 61, 155-158.
• 9. Levy J. K., Miller L. A., Crawford P. C., Ritchey J. W., Ross M. K., Fagerstome K. A.: GnRH immunocontraception of male cats. Theriogenology 2004, 62, 1116-1130.
• 10. Max A., Grabiec A., Garncarz M., Romanowicz-Barcikowska K.: Pobieranie nasienia kotów metodą elektroejakulacji. Medycyna Wet. 2004, 60, 1307-1311.
• 11. Morais R. N., Mucciolo R. G., Gomes M. L. F., Lacerda O., Moraes W., Moreira N., Graham L. H., Swanson W. F., Brown J. L.: Sesonal analysis of semen characteristics, serum testosterone and fecal androgens in the ocelot (Leopardus pardalis), margay (L. wiedli) and tigrina (L. tigrinus). Theriogenology 2002, 57, 2027-2041.
• 12. Pineda M. H., Dooley M. P., Martin P. A.: Long-term study on the effects of electroejaculation on seminal characteristics of the domestic cat. Am. J. Vet. Res. 1984, 45, 1038-1041.
• 13. Pukazhenthi B. S., Neubauer K., Jewgenow K., Howard J., Wildt D. E.: The impact and potential etiology of teratospermia in the domestic cat and its wild relatives. Theriogenology 2006, 66, 112-121.
• 14. Pukazhenthi B., Spindler R., Wildt D., May Bush L, Howard J.: Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: influence of adding and removing cryoprotectant. Cryobiology 2002, 44, 288-300.
• 15. Roth T. L., Swanson W. F., Blumer E., Wildt D. E.: Enhancing zona penetration by spermatozoa from a teratospermic species, the cheetah (Acinonyx jubatus). J. Exp. Zool. 1995, 271, 323-330.
• 16. Terio K. A., Marker L., Munson L.: Evidence for chronic stress in captive but not free-ranging cheetahs (Acinonyx jubatus) based on adrenal morphology and function. J. Wildl. Dis. 2004, 40, 259-266.
• 17. Wildt D. E., Bush M., Howard J. G., O'Brien S. J., Meltzer D., Van Dyk A., Ebedes H., Brand D. J.: Unique seminal guality in the South African cheetah and a comparative evaluation in the domestic cat. Biol. Reprod. 1983, 29, 1019-1025.
• 18. Zambelli D., Cunto M.: Semen collection in cats: Techniques and analysis. Theriogenology 2006, 66, 159-165.
• 19. Zambelli D., Cunto M., Prati F., Merlo B.: Effects of ketamine or medetomidine administration on quality of electroejaculated sperm and on sperm flow in the domestic cat. Theriogenology 2007, 68, 796-803.