Miscoding properties of isoguanine [2-oxoadenine] studied in an AMV reverse transcriptase in vitro system

• Autorzy: Bukowska A. M. , Kusmierek J.T.
• Języki publikacji: EN

Abstrakty

EN

We have found that isoguanine (¡G) can pair with thymine (iG T) and the non- natural base, 5-methylisocytosine (iG x iCM) during template directed synthesis catalyzed by AMV reverse transcriptase. The ratio of these pairings is about 1:10, irrespectively which of the templates, poly(C,iG) or poly(I,iG) is used. This ratio corresponds to the ratio of 2-OH and 2-keto tautomers in monomer in aqueous sol ution and apparently it is not influenced by the template context. Our results indicate also that formation of the reverse transcriptase catalyzed base pairs between iG and A, G or C can occur only at a low frequency, comparable to the frequency, of mismatches of.

Słowa kluczowe

EN

5-methylisocytosine; thymine; reverse transcriptase; oxidative damage; DNA base; isoguanine; in vitro; Croton bean; Croton tiglium; 2-oxoadenine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 1
• Opis fizyczny: p.247-254,fig.

Twórcy

autor

Bukowska A. M.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Kusmierek J.T.

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