Agreement between ELISA and complement fixation test used for diagnosing of paratuberculosis in goats

• Autorzy: Kaba J. , Gerlach G.F. , Nowicki M. , Rypula K.
• Języki publikacji: EN

Abstrakty

EN

An ELISA with a iipoarabinomannan as an antigen, developed for diagnosis of bovine paratuber- culosis, has been adapted for use in goats, and compared with complement fixation test. Kappa value of 0.62 indicated good agreement between CFT and the adapted ELISA and proved that the investigated ELISA may be helpful in diagnosis of Mycobacterium avium subsp. paratuberculosis infection in goats. The ELISA has been used to screen a randomly selected representative sample of Polish breeding goat population (21.78% of herds, 21.33% of goats). It has been demonstrated that only 2.42% of animals coming from 15.79% of herds were seropositive. Within-herd seroprevalence varied from 1.69% to 38.10%. Most of the infected animals (67.07%) were 3-4 years old. No seropositive cases were found in group up to 1 year old animals.

Słowa kluczowe

EN

paratuberculosis; Johne's disease zob.paratuberculosis; chronic disease; ruminant; goat; epidemiology; animal disease; complement fixation test; ELISA test; diagnosis; Mycobacterium avium ssp.paratuberculosis; Polish breeding; infection

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2008
• Tom: 11
• Numer: 3
• Opis fizyczny: p.209-212,fig.,ref.

Twórcy

autor

Kaba J.

• Warsaw Agricultural University, Nowoursynowska 159c, 02-787 Warsaw, Poland

autor

Gerlach G.F.

autor

Nowicki M.

autor

Rypula K.

Bibliografia

• Basu S, Basu A (1995) Comparison of several goodness-of-fit tests for the kappa statistic based on exact power and coverage probability. Stat Med 14: 347-356.
• Eamens GJ, Walker DM, Porter NS, Fell SA (2007) Pooled faecal culture for the detection of Mycobacterium avium subsp. paratuberculosis in goats. Aust Vet J 6: 243-251.
• Gorrie CJR (1959) John’s disease with particular reference to diagnosis. Laboatory aspects. Aust Vet J 35: 53-58.
• Hilbink F, West DM, Lisle de GW, Kittelberger R, Hosie BD, Hutton J, Cooke MM, Penrose M (1994) Compari­son of a complement fixation test, a gel diffusion test and two absorbed and unabsorbed ELISAs for diagnosis of paratuberculosis in sheep. Vet Microbiol 41: 107-116.
• Jark U, Ringena I, Franz B, Gerlach GF, Beyerbach M, Franz B (1997) Development of an ELISA technique for diagnosis of bovine paratuberculosis. Vet Microbiol 57: 189-198.
• Munjal SK, Boehmer J, Beyerbach M, Strutzberg-Minder K, Homuth M. (2004) Valuation of a LAM ELISA for diag­nosis of paratuberculosis in sheep and goats. Vet Micro­biol 3-4: 107-114.
• Munjal SK, Tripathi BN, Paliwal OP (2005) Progressive im- munopathological changes during early stages of experi­mental infection of goats with Mycobacterium avium sub­species paratuberculosis. Vet Pathol 4: 427-36.
• Rajukumar K, Tripathi BN, Kurade NP, Parihar NS (2001) An enzyme-linked immunosorbent assay using im- munoaffinity-purified antigen in the diagnosis of caprine paratuberculosis and its comparison with conventional ELISAs. Vet Res Commun 25: 539-553.
• Salgado M, Kruze J, Collins MT (2007) Diagnosis of para­tuberculosis by fecal culture and ELISA on milk and serum samples in two types of Chilean dairy goat herds. J Vet Diagn Invest 1: 99-102.
• Stewart DJ, Vaughan JA, Stiles PL, Noske PJ, Tizard ML, Prowse SJ, Michalski WP, Butler KL, Jones SL (2006) A long-term study in Angora goats experimentally infec­ted with Mycobacterium avium subsp. paratuberculosis: clinical disease, faecal culture and immunological studies. Vet Microbiol 1-2:13-24.
• Storset AK, Berg I, Djznne B. (2005) Evaluation of the gamma interferon test for diagnosis of paratuberculosis in goats. Vet Immunol Immunopathol 1-2: 87-94.
• Storset AK, Hasvold HJ, Valheim M, Brun-Hansen H, Be- rntsen G, Whist SK, Djznne B, Press CM. Holstad G, Larsen HJ (2001) Subclinical paratuberculosis in goats following experimental infection. An immunological and microbiological study. Vet Immunol Immunopathol 3-4: 271-287.
• Thrusfield M (2007) Veterinary Epidemiology. 3 rd ed., Blackwell Science, Oxford, England, pp. 57-66.