Zastosowanie metody Etest do oznaczania lekowrazliwych bakterii beztlenowych

• Autorzy: Rokosz A. , Sawicka-Grzelak A. , Kot K. , Meszaros J. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

Oznaczono lekowrażliwość 100 szczepów bakterii beztlenowych metodą E- test. Zastosowano paski Etest zawierające klindamycynę, metronidazol, piperacylinę, piperacylinę z tazobaktamem oraz imipenem. Określono wartości MIC każdego leku dla wszystkich szczepów. Wykryto po 10 szczepów opornych na klindamycynę, piperacylinę i imipenem. Siedem szczepów było opornych na metronidazol, a dwa szczepy na piperacylinę skojarzoną z tazobaktamem.

EN

The aim of this study was to evaluate Etest usefulness for antimicrobial susceptibility testing of obligate anaerobes and to compare the activity of five antibacterial drugs against clinical strains of anaerobes. One hundred strains of obligate anaerobes were tested: 2 reference strains (B. fragilis ATCC 25285 and B. thetaiotaomicron ATCC 29741) and 98 clinical strains isolated from patients of the Infant Jesus Clinical Hospital - Center for Trauma Treatment in Warsaw during the last three years (1997-1999). Strains of seven genera of obligate nonsporeforming anaerobes (Bacteroides, Prevotella, Porphyromonas, Fusobacterium, Peptostreptococcus, Propionibacterium and Actinomyces) and strains of two sporeforming species (C. perfringens and C. difficile) were examined. The MIC values were determined by the gradient diffusion method Etest (AB BIODISK, Sweden). Wilkins-Chalgren solid medium supplemented with 5% of sheep blood was used. Test plates were incubated at 35°C for 48 hours in glove-box (85% N2, 10% H2, 5% C02). The MIC values for each strain and antimicrobial agent, and the MIC ranges for bacteria of the same species were established. Ten strains resistant to clindamycin, ten resistant to piperacillin, and ten resistant to imipenem were detected. Seven strains were resistant to metronidazole and two strains to piperacillin combined with tazobactam. Tazobactam restored the susceptibility of eight strains to piperacillin. Obtained results confirm that Etest method is useful for antimicrobial susceptibility testing of obligate anaerobes. Older (clindamycin and metronidazole) and newer (piperacillin, piperacillin/tazobactam and imipenem) antimicrobial agents revealed high and comparable activity against clinical strains of obligate anaerobes. The percentage of strains susceptible to tested antimicrobials was ≥ 90. These antimicrobials may be still useful in the empiric treatment of infections caused by medically important anaerobes.

Słowa kluczowe

PL

wrazliwosc na leki; opornosc na metronidazol; bakterie beztlenowe; mikrobiologia lekarska; metoda Etest; opornosc na piperacyline; opornosc na klindamycyne; oznaczanie; opornosc na imipenem

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 2
• Opis fizyczny: s.167-175,tab.,bibliogr.

Twórcy

autor

Rokosz A.

• Akademia Medyczna, 02-004 Warszawa, ul.T.Chalubinskiego 5

autor

Sawicka-Grzelak A.

autor

Kot K.

autor

Meszaros J.

autor

Luczak M.

Bibliografia

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