Effect of detergents on xanthan production during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459

• Autorzy: Janas P , Gustaw W. , Mleko S. , Pielecki J.

Warianty tytułu

PL

Wpływ detergentów na produkcję ksantanu przez Xanthomonas campestris NRRL B-1459 podczas hodowli okresowych i ciągłych

• Języki publikacji: EN

Abstrakty

EN

The effect of the addition of detergents Tween 20, Tween 40, Tween 80 and Triton X-100 on xanthan production was investigated during batch and continuous cultivation of Xanthomonas campestris NRRL B-1459. Three of the four tested detergents: Tween 20, 40 and Triton-X 100 gave an increased xanthan production in comparison to control cultivation without detergent in the medium. The best results were achieved either with 0.05% or 0.1% concentrations of Triton-X 100. About 1.18 to 1.21 fold higher production of polymer was observed during batch cultivation in the presence of this compound in the medium. The highest xanthan concentrations were observed on day 5 and 4 of continuous cultivation in the presence of 0.05% and 0.1% of Triton X-100 (respectively 1.34 and 1.36 fold higher than control). Toxic effect of 0.1% Triton X-100 on the cells growth of the strain was observed after 5 days of continuous cultivation. In addition many examples of effect of detergents on the production of biotechnological useful compounds by various microorganisms have been presented in the work.

PL

Zbadano wpływ dodatku detergentów: Tweenu 20, Tweenu 40, Tweenu 80 i Tritonu X-100 na produkcję ksantanu przez Xanthomonas campestris NRRL B-1459 podczas hodowli okresowych i ciągłych. Trzy spośród czterech testowanych detergentów: Tween 20, 40 i Triton X-100 wpływały na zwiększenie produkcji ksantanu w porównaniu z kontrolną hodowlą okresową bez dodatku detergentu do podłoża. Najlepsze wyniki uzyskano stosując Triton X-100 w stężeniach 0,05% i 0,1%. W obecności tego związku w podłożu hodowlanym zaobserwowano zwiększenie produkcji polimeru podczas hodowli o prawie 1,18 i 1,21 raza. Najwyższe stężenia ksantanu oznaczono w 5 i 4 dniu hodowli ciągłej w obecności 0,05% i 0,1% Tritonu X-100 (odpowiednio 1,34 i 1,36 razy wyższe od kontroli). Stwierdzono toksyczny wpływ 0,1-procentowego Tritonu X-100 na wzrost komórek szczepu w drugiej części (od 5 dnia) hodowli ciągłej. Dodatkowo w pracy przedstawiono wiele przykładów wpływu detergentów na produkcję przez różne mikroorganizmy biotechnologicznie użytecznych substancji.

Słowa kluczowe

EN

production; detergent; Gram-negative bacterium; xanthan gum; Tween 40 detergent; Xanthomonas campestris; Tween 80 detergent; continuous cultivation; Triton X-100 detergent; Tween 20 detergent; xanthan

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2003
• Tom: 02
• Numer: 1
• Opis fizyczny: p.125-133,fig.

Twórcy

autor

Janas P

• University of Agriculture, Skromna 7, 20-704 Lublin, Poland

autor

Gustaw W.

autor

Mleko S.

autor

Pielecki J.

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