Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

• Autorzy: Slusarkiewicz-Jarzina A , Ponitka A
• Języki publikacji: EN

Abstrakty

EN

Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively.

Słowa kluczowe

EN

plant regeneration; microspore; triticale; haploid plant; anther culture; embryonic structure; androgenesis; somatic embryo

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 1997
• Tom: 38
• Numer: 3
• Opis fizyczny: p.253-258,fig.

Twórcy

autor

Slusarkiewicz-Jarzina A

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

autor

Ponitka A

• Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, Poland

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