Application of heminested RT-PCR to the detection of EBLV1 and classical rabies virus infections in bats and terrestrial animals

• Autorzy: Smreczak M , Orlowska A. , Trebas P. , Zmudzinski J.F.
• Języki publikacji: EN

Abstrakty

EN

The paper describes application and optimisation of hnRT-PCR in the detection of the fragment of nucleoprotein gene of Lyssaviruses (genotypes 1 and 5) as a method for laboratory diagnosis of rabies in terrestrial animals and bats. The heminested RT-PCR, because of its sensitivity and rapidity, it may be used as a technique for rabies diagnosis. The method can be applied both in living animals as well as in the case of post mortem collected samples. It provides not only rapid detection of rabies virus but also gives the material for sequencing of the PCR products for final identification of origin of the strain and epidemiological analysis.

Słowa kluczowe

EN

polymerase chain reaction; classical virus; diagnosis technique; veterinary medicine; EBLV1 virus; bat; rabies; Lyssavirus; terrestrial animal; rabies virus; laboratory diagnosis; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 1
• Opis fizyczny: p.15-18,fig.,ref.

Twórcy

autor

Smreczak M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Orlowska A.

autor

Trebas P.

autor

Zmudzinski J.F.

Bibliografia

• 1. Bourhy H., Kissi B., Audry L., Smreczak M., Sadkowska-Todys M., Kulonen K., Tordo N., Żmudziński J.F., Holmes E.C.: Ecology and evolution of rabies virus in Europe. J Gen Virol 1999, 80, 2545-2557.
• 2. Bourhy H., Kissi B., Tordo N.: Molecular diversity of the lyssavirus genus. Virology 1993, 194, 70-81.
• 3. Brookes S.M., Klopfleisch R., Müller T., Healy D.M., Teifke J.P., Lange E., Kliemt J., Johnson N., Johnson L., Kaden V., Vos A., Fooks A.R.: Susceptibility of sheep to European bat lyssavirus type-1 and -2 infection: a clinical pathogenesis study. Vet Microbiol 2007, 125, 210-223.
• 4. Crepin P., Audry L., Rotivel Y., Gacoin A., Caroff C., Bourhy H.: Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid. J Clin Microbiol 1998, 36, 1117-1121.
• 5. David D., Yakobson B., Rotenberg D., Dveres N., Davidson I., Stram T.: Rabies virus detection by RT-PCR in decomposed naturally infected brains. Vet Microbiol 2008, 87, 111-118.
• 6. Davis P.L., Holmes E.C., Larrous F., Van der Poel W.H.M., Tjørnehøj K., Alonso W.J., Bourhy H.: Phylogeography, population dynamics, and molecular evolution of European bat Lyssaviruses. J Virol 2005, 79, 10487-10497.
• 7. Fooks A.R., McElhinney L.M., Pounder D.J., Finnegan Ch.J., Mansfield K., Johnson N., Brookes S.M., Parsons G., White K., McIntyre P.G., Nathwani D.: Case report: isolation of a European Bat Lyssavirus type 2a from a fatal human case of a rabies encephalitis. J Med Virol 2003, 71, 281-289.
• 8. Hanlon C.A., Kuzmin I.V., Blanton J.D., Weldon W.C., Manangan J.S., Rupprecht C.E.: Efficacy of rabies biologies against new Lyssaviruses from Eurasia. Virus Res. 2005, 111, 44-54.
• 9. Harris S.L., Brookes S.M., Jones G., Hutson A.M., Racey P.A., Aegerter J., Smith G.C., McElhinney L.M., Fooks A.R.: European bat Lyssaviruses: distribution, prevalence and implications for conservation. Biol Conserv 2006, 131, 193-210.
• 10. Heaton P.R., Johnstone P., McElhinney L.M., Cowley R., O'sullivan E., Whitby J.E.: Heminested PCR assay for detection of six genotypes of rabies and rabies-related viruses. J Clin Microbiol 1997, 35, 2762-2766.
• 11. OIE, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris, 2004.
• 12. Picard-Meyer E., Bruyére V., Barrat J., Tissot E., Barrat M.J., Cliquet F.: Development of a hemi-nested RT-PCR method for the specific determination of European Bat Lyssavirus 1 Comparison with other rabies diagnostic methods. Vaccine 2004, 22, 1921-1929.
• 13. Smreczak M., Trębas P., Żmudziński J. F.: Rabies in Poland in 2006. Medycyna Wet 2008, 64, 68-71.
• 14. World Health Organization. WHO Expert Consultation on Rabies, Technical Report Series. First Report, No. 931. Geneva, Switzerland, World Health Organization, 2005.
• 15. Vázquez-Morón S., Avellón A., Echevarría J.E.: RT- PCR for detection of all seven genotypes of Lyssavirus genus. J. Virol. Methods, 2006, 135, 281 -287.