Developmental expression of the lipoprotein lipase gene in porcine subcutaneous adipose tissue

• Autorzy: Shan T. Z. , Wang Y.Z. , Liu Y.J. , Liu J.X. , Feng J. , Xu Z.R.
• Języki publikacji: EN

Abstrakty

EN

Lipoprotein lipase (LPL) has been implicated as a key enzyme in lipid metabolism. The pattern of LPL gene expression in different ages and its relation to adipose deposition was studied. Fifteen female Duroc × Landrace × Yorkshire pigs aged at 1 day, 7, 14, 21 and 28 weeks (three pigs per age, 10% per each bred) were used to study developmental gene expression of LPL in subcutaneous adipose tissue by means of semi-quantitative RT-PCR. The results showed that the LPL mRNA levels in subcutaneous adipose tissue significantly increased from 1 day to 7 weeks (P<0.01), and then gradually decreased from 7 to 28 weeks. A strong inverse correlation between LPL mRNA levels and age as well as adipose index and body weight was found between 7 and 28 weeks. Body weight and total adipose weight increased significantly with age (P<0.01).

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2006
• Tom: 15
• Numer: 4
• Opis fizyczny: p.621-629,fig.,ref.

Twórcy

autor

Shan T. Z.

• Zhejiang University, No. 164 Quitao North Road, Hangzhou 310029, P.R. China

autor

Wang Y.Z.

autor

Liu Y.J.

autor

Liu J.X.

autor

Feng J.

autor

Xu Z.R.

Bibliografia

• Adachi H., Kurachi H., Homma H., Adachi K., Tmai T., Sakata M., Matsuzawa Y., Miyake A., 1995. Invovement of epidermal growth factor in inducing adiposity of age female mice. J. Endocrinol. 146, 381-393
• Barber M.C., Ward R.J., Richards S.E., Salter A.M., Buttery P.J., Vernon R.G., Travers M.T., 2000. Ovine adipose tissue monounsaturated fat content is correlated to depot-specific expression of the stearoyl-CoA desaturase gene. J. Anim. Sci. 78, 62-68
• Bey L., Areiqat E., Sano A., Hamilton M.T., 2001. Reduced lipoprotein lipase activity in the postural skeletal muscle during aging. J. Appl. Physiol. 91, 687-692
• Bonnet M., Leroux C., Faulconnier Y., Hocquette J.F., Bocquier F., Martin P., Chilliard Y., 2000. Lipoprotein lipase activity and mRNA are up-regulated by refeeding in adipose tissue and cardiac muscle of sheep. J. Nutr. 130, 749-756
• Eckel R., 1989. Lipoprotein lipase: a multifunctional enzyme relevant to common metabolic diseases. N. Engl. J. Med. 320, 1060-1068
• Hocquette J.F., Graulet B., Olivecrona T., 1998. Lipoprotein lipase activity and mRNA levels in bovine tissues. Comp. Biochem. Physiol. Pt. B 121, 201-212
• Hotta K., Bodkin N.L., Gustafson T.A., Yoshioka S., Ortmeyer H.K., Hansen B.C., 1999. Age-related adipose tissue mRNA expression of ADD1/SREBP1, PPARg, lipoprotein lipase, and GLUT4 glucose transporter in rhesus monkeys. J. Gerontol. A. Biol. Sci. Med. Sci. 54, 183-188
• Imbeault P., Prudhome D., Tremblay A., Despres J.P., Mauriege P., 2000. Adipose tissue metabolism in young and middle-aged men after control for total body fatness. J. Clin. Endocrinol. Metab. 85, 2455-2462
• Imbeault P., Vidal H., Tremblay A., Vega N., Nadeau A., Despres J.P., Mauriege P., 2001. Agerelated differences in messenger ribonucleic acid expression of key proteins involved in adipose cell differentiation and metabolism. J. Clin. Endocrinol. Metab. 86, 828-833
• Maingrette F., Renier G., 2003. Leptin increases lipoprotein lipase secretion by macrophages: Involvement of oxidative stress and protein kinase C. Diabetes 52, 2121-2128
• Marone M., Mozzetti S., Ritis D.D., Pierelli L., Scambia G., 2001. Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample. Biol. Procedures Online 3,19-25
• Merkel M., Eckel R.H., Goldberg I.J., 2002. Lipoprotein lipase: genetics, lipid uptake, and regulation. J. Lipid Res. 43, 1997-2006
• Mersmann H.J., 1998. Lipoprotein and hormone-sensitive lipase in porcine adipose tissue. J. Anim. Sci. 76, 1396-1404
• Mooradian A.D., Albert S.G., 1999. The age-related changed in lipogenic enzyme: the role of dietary factors and thyroid hormone responsiveness. Mech. Age. Dev. 108, 139-149
• Nogalska A., Swierczynski J., 2001. The age-related differences in obese and fatty acid synthase gene expression in white adipose tissue of rat. Biochim. Biophys. Acta 1533, 73-80
• Pethick D.W., Dunshea F.R., 1993. Fat metabolism and turnover. In: J.M. Forbes, J. France (Editors). Quantitative Aspects of Ruminant Digestion and Metabolism. CAB International Press, Wallingford, pp. 291-311
• Ren M.Q., Wegner J., Bellmann O., Brockmann G.A., Schneider F., Teuscher F., Ender K., 2002. Comparing mRNA levels of genes encoding leptin, leptin receptor, and lipoprotein lipase between dairy and beef cattle. Domest. Anim. Endocrinol. 23, 371-381
• SAS, 1989. SAS User’s Guide. SAS Institute Inc., Cary, NC Schwartz R.S., 1998. Obesity in the elderly. In: G.A. Bray, C. Bouchard, W.P.T. James (Editors).
• Handbook of Obesity. Marcel Dekker, Inc. New York, pp. 103-114
• Souza D.N., Pethick D.W., Dunshea F.R., Suster D., Pluske J.R., Mullan B.P., 2004. The pattern if fat and lean muscle tissue deposition differs in the different pork primal cuts of female pigs during the finisher growth phase. Livest. Prod. Sci. 91, 1-8
• Taylor B.A., Phillips S.J., 1996. Detection of obesity QTLs on mouse chromosomes 1 and 7 by selective DNA pooling. Genomics 34, 389-398
• Ursini F., Vugman M., Fernandes L.C., Curi C., Curi R., 1991. Metabolic changes of several adipose depots as caused by aging. Physiol. Behav. 50, 317-321