Extraction and chromatographic separation of tannin fractions from tannin-rich plant material

• Autorzy: Karamac M. , Kosinska A. , Rybarczyk A. , Amarowicz R.

Warianty tytułu

PL

Ekstrakcja i chromatograficzne wyodrebnianie frakcji taninowych z bogatego w taniny materialu roslinnego

• Języki publikacji: EN

Abstrakty

EN

The crude phenolic extracts were obtained from red bean, adzuki bean, red lentil, green lentil, broad bean, faba bean, field bean, vetch, buckwheat, buckwheat groat, hazelnuts, walnuts, and almonds using 80% (v/v) acetone. The tannin fractions were separated from the crude extracts using Sephadex LH-20 column chromatography with ethanol and 50% (v/v) acetone. The tannin fractions were characterised by colour reaction with vanillin/HCl reagent and ability to bovine serum albumin (BSA) precipitation. The yield of extraction of phenolic compounds ranged from 3.77% (buckwheat) to 17.20% (walnuts). The content of tannin fraction in the crude extracts was differentiated and ranged from 10.5% (faba bean) to 45.5% (broad bean). The tannin fraction from buckwheat possessed the highest content of tannins determined using vanillin method (1.04 absorbance unit at 500 nm/mg) whereas the lowest content was noted for broad bean (0.066 absorbance unit at 500 nm/mg). In the model system, the highest amount of BSA was precipitated by tannin fraction of hazelnuts, whereas the lowest was noted for adzuki bean.

PL

Surowe ekstrakty związków fenolowych uzyskano z czerwonej fasoli, fasoli adzuki, zielonej i czerwonej soczewicy, bobu, bobiku, peluszki, wyki, gryki, kaszy gryczanej, orzechów laskowych, orzechów włoskich i migdałów stosując 80% (v/v) aceton. Frakcje taninowe wyodrębnione z ekstraktów na drodze chromatografii kolumnowej na żelu Sephadex LH-20 z etanolem i 50% (v/v) acetonem scharakteryzowano reakcją barwną z odczynnikiem wanilinowym oraz poprzez zdolność do precypitacji albuminy surowicy bydlęcej (BSA). Ekstrakcyjność wahała się od 3,77% (gryka) do 17,20% (orzech włoski). Zawartość frakcji taninowej w surowym ekstrakcie wahała się od 10,50% (bobik) do 45,50% (bób). Posługując się metodą wanilinową największą zawartość tanin stwierdzono we frakcji acetonowej z gryki (1.04 jednostki absorbancji przy 500 nm/mg), najniższą w przypadku bobu (0.066 jednostki absorbancji przy 500 nm/mg). W badaniach modelowych najwyższą zdolnością do precypitacji BSA charakteryzowała się frakcja taninowa z orzechów laskowych, najniższą zdolność zanotowano w przypadku frakcji taninowej z fasoli adzuki.

Słowa kluczowe

EN

plant material; leguminous seed; nut; buckwheat; extraction; separation; chromatography; column chromatography; tannin; tannin-rich plant material; protein precipitating capacity

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2007
• Tom: 57
• Numer: 4
• Opis fizyczny: p.471-474,fig.,ref.

Twórcy

autor

Karamac M.

• Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland

autor

Kosinska A.

autor

Rybarczyk A.

autor

Amarowicz R.

Bibliografia

• 1. Alasalvar C., Karamać M., Amarowicz R., Shahidi F., Antioxidant and antiradical activities in extracts of hazelnut kernel (Corylus avellan L.) and hazelnut green leafy cover. J. Agric. Food Chem., 2006, 54, 4826–4832.
• 2. Amarowicz R., Tannins: the new natural antioxidants? Eur. Lipid Sci. Technol., 2007, 109, 549–551.
• 3. Amarowicz R., Piskuła M., Honke J., Rudnicka B., Troszyńska A., Kozłowska H., Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol. J. Food Nutr. Sci., 1995, 45, 53–62.
• 4. Amarowicz R., Naczk M., Shahidi F., Zadernowski R., Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose, and faba bean. J. Food Lipids, 2000, 7, 195–205.
• 5. Amarowicz R., Troszyńska A., Baryłko-Pikielna N., Shahiid F., Polyphenolics extracts from legume seeds: correlation between total antioxidant activity, total phenolics content, tannins content and astringency. J. Food Lipids, 2004, 11, 278–286.
• 6. Amarowicz R., Estrella I., Hernández T., Troszyńska A., Antioxidant activity of tannin fractions from nuts. Eur. J. Lipid Sci. Technol., 2007, submitted.
• 7. Ariga T., Koshiyama I., Fukushima D., Antioxidative properties of procyanidins B-1 and B-3 from adzuki beans in aqueous system. Agric. Biol. Chem., 1988, 52, 2717–2722.
• 8. Ariga T., Hamano M., Radical scavenging action and its mode in procyanidins B-1 and B-3 from adzuki beans to peroxyl radicals. Agric. Biol.Chem., 1990, 54, 2499–2504.
• 9. Awika J.M., McDonough L.W., Rooney L.W., Decorticating sorghum to concentrate healthy phytochemicals. J. Agric. Food Chem., 2006, 53, 6230–6234.
• 10. Bate-Smith E.C., Swein T., Flavonoid compounds. 1962, in: Comparative Biochemistry (eds. H.S. Mason, A.M. Florkin), Academic Press, New York, pp. 755–809.
• 11. Chavan U.D., Amarowicz R., Shahidi F., Antioxidative activity of phenolic fractions of beach pea (Lathyrus maritimus L.). J. Food Lipids, 1999, 6, 1–11.
• 12. Chung K.-T., Wei C.-I., Johnson M.G., Are tannins a double- -edged sword in biology and health? Trends Food Sci. Nutr., 1998, 9, 168–175.
• 13. Cos T., De Bruyne N., Hermans S., Apers D.V., Berghe A., Vlietinck J., Proanthocyanidins in health care: Current and new trends. Current Med. Chem., 2004, 11, 1345–1359.
• 14. De Pascual T.S., Santos-Buelga C., Rivas-Gonzalo J.C., Quantitative analysis of flavan 3-ols in Spanish foodstuffs and beverages. J. Agric. Food Chem., 2000, 48, 5331–5337.
• 15. Dueñas M., Estrella I., Hernández T., Phenolic composition of the cotyledon and the seed coat of lentil (Lens culinaris L.). Eur. Food. Res. Technol., 2002, 215, 478–483.
• 16. Dueñas M., Sun B.A., Hernández T., Estrella I., Sparanger I., Proanthocyanidins composition in the seed coat of lentils (Lens culinaris L.). J. Agric. Food Chem., 2003, 51, 7999–8004.
• 17. Dueñas M., Estrella I., Hernández T., Occurrence of phenolic compounds in the seed coat and the cotyledon of pea (Pisum sativum L.). Eur. Food. Res. Technol., 2004, 219, 116–123.
• 18. Durling N.E., Catchpole O.J., Grey J.B., Webby R.F., Mitchell K.A., Foo L.Y., Perry N.B., Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixture. Food Chem., 2007, 101, 1417–1424.
• 19. Hagerman A.E., Butler L.G., Protein precipitation method for the quantitative determination of tannins. J. Agric. Food Chem., 1978, 26, 809–812.
• 20. Hagerman A.E., Zhao Y., Johnson S., Methods for determination of condensed and hydrolysable tannins. ACS Symp. Series, 2005, 662, 209–222.
• 21. Naczk M., Oickle D., Pink D., Shahidi F., Protein precipitating capacity of crude canola tannins: Effect of pH, tannin, and protein concentrations. J. Agric. Food Chem., 1996, 44, 2144– 2148.
• 22. Naczk M., Amarowicz R., Zadernowski R., Shahidi F., Proteinprecipitating capacity of crude condensed tannins of canola and rapeseed hulls. J. Am. Oil Chem. Soc., 2001, 78, 1173–1178.
• 23. Naczk M., Amarowicz R., Zadernowski R., Shahidi F., Antioxidant capacity of phenolics from canola hulls as affected by different solvents. ACS Symp. Series, 2005, 909, 57–66.
• 24. Naczk M., Grant S., Zadernowski R., Barre E., Protein precipitating capacity of phenolics of wild blueberry leaves and fruits. Food Chem., 2006, 96, 640–647.
• 25. Naczk M., Zadernowski R., Shahidi F., Antioxidant capacity of phenolic extracts from selected food by-products. ACS Symposium Series, 2007, 956, 184–185.
• 26. Pegg R.B., Amarowicz R., Nacz. M., Shahidi F., PHOTOCHEM for determination of antioxidant capacity of plant extracts. ACS Symposium Series, 2007, 956, 140–158.
• 27. Price M.L, Van Scoyoc S., Butler L.G., A critical evaluation of the vanillic reaction as an assay for tannin in sorghum grain. J. Agric. Food Chem., 1978, 26, 1214–1218.
• 28. Rocha-Guzmán N.E., Herzon A., Gonzáles-Laredo R.F., Ibarra- Pérez F.J., Zambrano-Galván G., Gallegos-Infante J.J., Antioxidant and antimutagenic activity of phenolic compounds in three different color groups of common bean cultivars (Phaseolus vulgaric). Food Chem., 2007, 103, 521–527.
• 29. Siddhuraju P., Becker K., The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extracts. Food Chem., 2007, 101, 10–19.
• 30. Troszyńska A., Honke J., Kozłowska H., Polyphenolic compounds in faba bean (Vicia faba) seed coat. 1993, in: Recent Advances of Research in Antinutrional Factors in Legume Seeds (eds. A.F.B. Van der Poel, J. Huisman, H.S. Saini). Wageningen Press, Wageningen, pp. 91–94.
• 31. Zieliński H., Kozłowska H., Antioxidant activity and total phenolics in selected cereal grains and their different morphological fractions. J. Agric. Food Chem., 2000, 48, 2008–2016.