Odkazanie eksplantatow buka [Fagus sylvatica L.] do kultur tkankowych

• Autorzy: Kraj W , Dolnicki A.
• Języki publikacji: PL

Abstrakty

EN

Ripe, stratified beechnuts, turned out to be almost impossible to sterilize employing the commonly used sterilizers. The recommended way of sterilizing beech nuts is to soak (for 5 minutes, in a 2% solution of NaOCl) the embryos with parts of cotyledons, isolated from nuts partly sterilized with an 15% solution of NaOCl for 15 minutes. The chloraminę (concentration 4%, time of soaking 2 minutes) provided 100% sterility of the fragments of epicotyles and buds; it also increased up to 80% the effectiveness of the sterilization of cotyledons of beech seedlings. The only way to obtain the required effectiveness of sterilization of buds collected in winter from 20-year old trees turned out to be soaking in HgCl2; its concentration and time of exploit soaking can be reduced in comparison to those proposed by Chalupa (1985a), namely from 0.1-0.3% and 15-30 minutes down to 0.05% and 30 seconds.

Słowa kluczowe

PL

lesnictwo; drzewa lesne; buk zwyczajny; Fagus sylvatica; eksplantaty; odkazanie; kultury tkankowe; kultury in vitro

EN

forestry; forest tree; European beech; Fagus sylvatica; explant; sterilization; tissue culture; in vitro culture

• Wydawca: -
• Czasopismo: Acta Agraria et Silvestria. Series Silvestris
• Rocznik: 1998
• Tom: 36
• Opis fizyczny: s.49-62,tab.,bibliogr.

Twórcy

autor

Kraj W

• Akademia Rolnicza, al.29 Listopada 46, 31-425 Krakow

autor

Dolnicki A.

Bibliografia

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