Invertase and alpha-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcticum

• Autorzy: Turkiewicz M , Pazgier M. , Donachie S.P. , Kalinowska H.
• Języki publikacji: EN

Abstrakty

EN

The marine psychrophilic and endemic Antarctic yeast Leucosporidium antarcticum strain 171 synthesizes intracellular ß-fructofuranosidase, and intra- and extracellular α-glucosidases. Each enzyme is maximally produced at 5°C, while the strain's optimum growth temperature is 15°C. Invertase biosynthesis appeared regulated by catabolic repression, and induced by sucrose; the enzyme was extremely unstable ex vivo, and only EDTA, Mn2+, and BSA stabilized it for up to 12 h after yeast cell lysis. Thermal stability of the invertase was also low (30 min at temperatures up to 12°C). The optimum temperature for invertase activity was 30°C, and optimum pH was 4.55 to 4.75. The extracellular α-glucosidase was maximally active at 35°C and pH 6.70-7.50, and stable for 30 min up to 20°C.

Słowa kluczowe

EN

invertase; alpha-glucosidase; production; endemic species; Antarctic; yeast; Leucosporidium antarcticum; microorganism

• Wydawca: -
• Czasopismo: Polish Polar Research
• Rocznik: 2005
• Tom: 26
• Numer: 2
• Opis fizyczny: p.125-136,fig.,ref.

Twórcy

autor

Turkiewicz M

• Technical University of Lodz, Stefanowskiego 4/10, Lodz, Poland

autor

Pazgier M.

autor

Donachie S.P.

autor

Kalinowska H.

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