NMR measurements of proton exchange between solvent and peptides and proteins

• Autorzy: Wojcik J. , Ruszczynska K. , Zhukov I. , Ejchart A.
• Języki publikacji: EN

Abstrakty

EN

Scope and limitations of the NMR based methods, equilibration and magnetization transfer, for measuring proton exchange rates of amide protons in peptides and proteins with water protons are discussed. Equilibration is applied to very slow processes detected by hydrogen-deuterium exchange after a solute is dissolved in D2O. Magnetization transfer allows to study moderately rapid processes in H2O. A number of precautions should be undertaken in order to avoid systemic errors inherent in the magnetization transfer method.

Słowa kluczowe

EN

solvent; proton exchange; peptide; measurement; magnetization transfer; protein; nuclear magnetic resonance; equilibration; hydrogen-deuterium exchange

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.651-663,fig.

Twórcy

autor

Wojcik J.

• Polish Academy of Sciences, A.Pawinskiego 5A, 02-106 Warsaw, Poland

autor

Ruszczynska K.

autor

Zhukov I.

autor

Ejchart A.

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