Wystepowanie i charakterystyka szczepow opornych na antybiotyki oksy-imino-betalaktamowe wsrod szczepow Salmonella enterica subsp. enterica izolowanych w Polsce

• Autorzy: Szych J. , Gierczynski R. , Wardak S. , Cieslik A.

Warianty tytułu

EN

The occurrence and characterisation of oxy-imino-beta-lactams resistant strains among Salmonella enterica subsp. enterica isolated in Poland

• Języki publikacji: PL

Abstrakty

PL

Scharakteryzowano 68 szczepów pałeczek Salmonella opornych na antybiotyki oksy-imino-betalaktamowe należących do 6 typów serologicznych, wybranych spośród 239 opornych na ampicylinę szczepów pałeczek Salmonella izolowanych w Polsce w latach 1999-2004 z próbek materialu klinicznego. Stwierdzono, że większość badanych szczepów wytwarzała p-Iaktamazę TEM-1 o pl=5,4 oraz p-laktamazę o rozszerzonym spektrum substratowym CTX-M-3 o pl=8,4. Wszystkie badane szczepy S. Thompson i jeden ze szczepów S. Typhimurium wytwarzał enzym SHV-5 o pl=8,2. Jest to pierwsze doniesienie o produkcji SHV-5 przez pałeczki Salmonella w Polsce.

EN

Extended - spectrum ß-lactamases (ESBLs) are enzymes manifesting considerable hydrolyzing activity on a wide variety of ß-lactam antibiotics including oxyiminocephalosporins and aztreonam. In the study reported here we investigated the types of ESBL produced by Salmonella enterica subsp. enterica strains isolated from clinical samples in the microbiological laboratories of sanitary-epidemiological units in Poland from 1999 to 2004. Among 239 ampicilline-resistant Salmonella enterica subsp. enterica strains isolated from clinical samples in the microbiological laboratories of sanitary-epidemiological units in Poland, 68 isolates of oximino-beta-lactams resistant of 6 serovars were found. There were 16 epidemiological unrelated strains (6 isolates of S.Enteritids, S isolates of S.Thompson, 3 isolates of S.Typhimurium, onefold isolate of S.Muenster and S. enterica 1,9,12:-:-) coming from different areas of country and 52 epidemiologically related isolates of S. Oranienburg, coming from a prolonged outbreak in an orphanage in Łódź. All the strains were identified as the ESBLs producers . The molecular analysis relevead that most of them expressed CTX-M-3 ESBL which is widely observed in Poland and additional enzyme TEM-1. All tested isolates of S.Thompson and one of three S. Typhimurium isolates were found to produce SHV-5 ESBL. This is the first report regarding the presence of SHV-5 in the genus Salmonella in Poland.

Słowa kluczowe

PL

Salmonella enterica subsp.enterica; Polska; wystepowanie; szczepy bakteryjne; izolacja; opornosc na antybiotyki; ampicylina; antybiotyki oksy-imino-beta-laktamowe; beta-laktamaza; lancuchowa reakcja polimerazy; analiza sekwencyjna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 2
• Opis fizyczny: s.115-130,rys.,tab.,bibliogr.

Twórcy

autor

Szych J.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Gierczynski R.

autor

Wardak S.

autor

Cieslik A.

Bibliografia

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