Characterization and protectivity of Acanthocheilonema viteae adult somatic proteins

• Autorzy: Singh D P , Misra S , Chatterjee R K
• Języki publikacji: EN

Abstrakty

EN

Soluble somatic extract of adult Acanthocheilonema viteae was resolved into three major peaks - Fr. I, Fr. II and Fr. III by gel-filtration on Sephadex G-200 column. Protein rich Fr. I was highly efficient in detecting haemagglutinating and precipitating antibodies while Fr. II, a carbohydrate rich fraction was reactive in detecting hom ocyt otropic antibodies. Fraction II also reacted feebly in precipitation test. Fraction III was though weakly but exclusively reactive in P-K test. On SDS-PAGE analysis Fr. I revealed 33 protein bands with molecular weights ranging from 25 to 100 kD. The 15 bands of Fr. II remained within the molecular weight range of 10 and 28 kD. Fraction III containing 9 protein components mostly within 17 kD when used as immunogen caused significant reduction in microfilaraemia (P<0.001) and lowered adult worm recovery (P<0.05) as compared to other fractions and control groups.

Słowa kluczowe

EN

somatic protein; Acanthocheilonema viteae; protein

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1992
• Tom: 37
• Numer: 3
• Opis fizyczny: p.145-150,fig.

Twórcy

autor

Singh D P

• Division of Parasitology, Central Drug Research Institute, P.O.Box 73, Lucknow 226001, India

autor

Misra S

• Division of Parasitology, Central Drug Research Institute, P.O.Box 73, Lucknow 226001, India

autor

Chatterjee R K

• Division of Parasitology, Central Drug Research Institute, P.O.Box 73, Lucknow 226001, India

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