Application of electrophoretic methods for detection of protein-porphyrin complexes

• Autorzy: Kowalska A. , Kwiek P. , Milosz E. , Gondek G. , Romiszewska A. , Graczyk A. , Podhajska A.J.
• Języki publikacji: EN

Abstrakty

EN

Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc™ 2000 system, and a system specially designed for the purpose of these investigations, concerning protein-porphyrin interactions. The fluorescent complexes can be electro-trans­ferred from the gel onto PVDF membrane, eluted and analyzed in order to identify the protein interacting with porphyrins.

Słowa kluczowe

EN

protein-porphyrin complex; therapy; photodynamic diagnosis; electrophoresis; photodynamic therapy; porphyrin

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 4
• Opis fizyczny: p.1155-1163,fig.,ref.

Twórcy

autor

Kowalska A.

• University of Gdansk, Gdansk, Poland

autor

Kwiek P.

autor

Milosz E.

autor

Gondek G.

autor

Romiszewska A.

autor

Graczyk A.

autor

Podhajska A.J.

Bibliografia

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