The isolation, partial purification and some characteristics of proteasomes from Trichostrongylus colubriformis larvae

• Autorzy: Hadas E , Stankiewicz M
• Języki publikacji: EN

Abstrakty

EN

Proteasomes or multicatalytic proteinases have recently been discovered in mammalian cells. These enzymes are major non-lysosomal proteinases of eukaryotic cells that can account up to 1% of the soluble cellular protein. Our previous studies indicated that multicatalytic proteinases are present in parasitic nematodes of sheep. In this study we have demonstrated that the method described by Rivett et al. (1994) and used for mammalian cells, can give reasonably good preparations of proteasomes from Trichostrongylus colubriformis infective larvae. Further, the inactivation of proteasomes activity by antisera suggests that enzymatic activity of proteasomes is recognised by the immune system of sheep.

Słowa kluczowe

EN

sheep; eukaryotic cell; immune system; cellular protein; enzyme activity; purification; Trichostrongylus colubriformis; larva; mammalian cell; parasitic nematode

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 1997
• Tom: 42
• Numer: 2
• Opis fizyczny: p.121-123,fig.

Twórcy

autor

Hadas E

• AgResearch, Wallaceville Animal Research Centre, P.O.Box 40063, Upper Hutt, New Zealand
• Department of Biology and Medical Parasitology, K. Marcinkowski University of Medical Sciences, Fredry 10, 61-701 Poznań, Poland

autor

Stankiewicz M

• AgResearch, Wallaceville Animal Research Centre, P.O.Box 40063, Upper Hutt, New Zealand

Bibliografia

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