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2007 | 56 | 3 |

Tytuł artykułu

Antibiotic susceptibility and molecular characterisation of Proteus mirabilis isolates of hospitals from the West Pomeranian area of Poland

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Proteus mirabilis isolates (n=177), collected between 1996 and 2000 in four hospitals in the West Pomeranian area of Poland, were characterized by antibiotype and pulsed-field gel electrophoresis (PFGE). The selected isolates were collected from different wards (intensive care unit, surgery, internal medicine, and urology). The strains were cultured from various specimen types, mostly from urine, wound samples, bronchial exudates and sputa. The identification was done by biochemical test ID 32E ATB (bioMerieux). Analysis of PFGE patterns was based on comparison of the banding patterns obtained by PFGE of chromosomal DNA digested with SHI enzyme. Among all P. mirabilis isolates tested three major genotypes A (A1-A7), B (B1-B4), C (C1-C5) and 71 unique patterns were identified. The same genotypes were obtained from different patients, treated in different wards and hospitals during a 5-year period. The strains which belonged to the genotypes A and B were multiresistant and most of them produced ESBL; genotype C was more sensitive to antibiotics.

Wydawca

-

Rocznik

Tom

56

Numer

3

Opis fizyczny

p.169-173,fig.,ref.

Twórcy

autor
  • Pomeranian Medical University, Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland

Bibliografia

  • Bonnet R., H. Marchandin, C. Chanat, D. Sirot, R. Labia, C. De Champs, E. Jumas-Bilak and J. Sirot. 2002. Chromosome-encoded class D ß-lactamase OXA-23 in Proteus mirabilis. Antimicrob. Agents Chemother. 46: 2004-2006.
  • Chippendale G.R., J.W. Warren and A.L.Trifillis. 1994. Internalization of Proteus mirabilis by human renal epithelial cells. Infect. Immun. 62: 3115-3121.
  • Clapham L., R.J.C. McLean and J.C. Nickel. 1990. The influence of bacteria on struvite crystal habit and its importance in urinary stone formation. J. Crystal. Growth 104: 475-484.
  • Fernandez-BacaV., F. Ballesteros, J.A. Hervas, P. Villalon, M.A. Domínguez, V.J. Benedi and S. Alberti. 2001. Molecular epidemiological typing of Enterobacter cloacae isolates from a neonatal intensive care unit: three-year prospective study. J. Hosp. Infect. 49: 173-182.
  • Fiet J., A. Pałucha, B. Maczyńska, M. Stankiewicz, H. Przondo-Mordarska, W. Hryniewicz and M. Gniadkowski. 2000. A novel complex mutant ß-lactamase, TEM-68, identified in a Klebsiella pneumoniae isolate from an outbreak of extended-spectrum ß-lactamase-producing Klebsiellae. Antimicrob. Agents Chemother. 44: 1499-1505.
  • Goering R.V. 1993. Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis. Infect. Control Hosp. Epidemiol. 14: 595-600.
  • Hennekinne J.A., A. Kerouanton, A. Brisabois and M.L. De Buyser. 2003. Discrimination of Staphylococcus aureus bio-types by pulsed-field gel electrophoresis of DNA macro-restriction fragments. J. Appl. Microbiol. 94: 321-329.
  • Johnson D.E., R.G. Russell, CV. Lockatell, J.C. Zulty, J.W. Warren and H.L.T. Mobley. 1993. Contribution oí Proteus mirabilis urease to persistence, urolithiasis, and acute pyelonephritis in a mause model of ascending urinary tract infection. Infect. Immun. 61: 2748-2754.
  • Mammeri H., L. Gilly, G. Laurans, G. Vedel, F. Eb and G. Paul. 2001. Catalytic and structural properties of IRT-21 ß-lactamase (TEM-77) from a co-amoxiclav- resistant Proteus mirabilis isolate. FEMS Microbiol. Lett. 205: 185-189.
  • Neuwirth C, S. Madec, E. Siebor, A. Pechinot, J.M. Duez, M. Pruneaux, M. Fouchereau-Peron, A. Kazmierczak and R. Labia. 2001. TEM-89 ß-lactamase produced by a Proteus mirabilis clinical isolate: new complex mutant (CMT 3) with mutations in both TEM-59 (IRT-17) and TEM-3. Antimicrob. Agents Chemother. 45: 3591-3594.
  • Pałucha A., B. Mikiewicz, W. Hryniewicz and M. Gniadkowski. 1999. Concurrent outbreaks of extended-spectrum ß-lactamase-producing organisms o the family Enterobacteriaceae in a Warsaw hospital. J. Antimicrob. Chemother. 44: 489-499.
  • Perilli M., B. Segatore, M.R. De Massis, N. Franceschini, C. Bianchi, G.M. Rossolini and G. Amicosante. 2002. Characterization of a new extended-spectrum ß-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey. Antimicrob. Agents Chemother. 46: 925-928.
  • Saladin M., V.T.B. Cao, T. Lambert, J.L. Donay, J.L. Herrmann, Z. Ould-Hocine, Ch. Verdet, F. Delisle, A. Philippon and G. Arlet. 2002. Diversity of CTX-M ß-lactamases and their promoter regions from Enterobacteriaceae isolated in three Parisian hospitals. FEMS Microbiol. Lett. 209: 161-168.
  • Tenover EC, R.D. Arbeit, R.V. Goering, P.A. Mickelsen, B.E. Murray, D.H. Persing and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J. Clin. Microbiol. 33: 2233-2239.
  • Toltzis P., M.J. Dul, C. Hoyen, A. Salvator, M. Walsh, L. Zetts and H. Toltzis. 2001. Molecular epidemiology of antibiotic-resistant Gram-negative bacilli in a neonatal intensive care unit during a nonoutbreak period. Pediatrics 108: 1143-1148.
  • Traub W.H., B. Leonhard and D. Bauer. 1996. Gentamicin- and methicillin-resistant Staphylococcus aureus: phenotypic and geno-typic characterization of three putative nosocomial outbreak strains. Chemotherapy 42: 21-36.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-e4be4667-e734-447e-87ec-342646652c3e
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