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2002 | 07 | 2B |

Tytuł artykułu

Tagging QTLs for maximum root length in rainfed lowland rice [Oryza sativa L.] using molecular markers

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
A number of morphological, physiological and phenological traits are known to improve the performance of rice challenged by drought. Root morphological traits and stress-induced response form important components of drought tolerance. Enhancing grain yield remains the principal objective of most breeding programs. Interaction between primary traits poses a formidable challenge while dealing with grain yield under stress. The evaluation of root morphology at three different growth stages and grain yield along with related characteristics under contrasting moisture regimes was made using nine backcrosses along with their parent and standard checks. The backcrosses invoved transgressant double haploid lines derived from IR64 and Azucena with IR64. Marked genotypic differences were observed for all root morphology as well as grain yield related characteristics across the sampling dates as revealed by individual and combined ANOVA. Among the nine backcrosses studied in this experiment, the BC1F2 population of P124 x IR64 were evaluated for forwarding based on their performance with respect to maximum root length and grain yield under both well-watered and low-moisture stress conditions. Sixtynine plants - ten percent of the backcross population - were selectively genotyped using RAPD primers. Under well-watered conditions two RAPD markers showed strong linkage to QTLs for maximum root length evaluated under ww conditions. Two other markers could explain the considerable amount of variation in MRL under LMS. One of the markers identified under low-moisture stress conditions was also able to explain variability in maximum root length in the mean environment.

Wydawca

-

Rocznik

Tom

07

Numer

2B

Opis fizyczny

p.771-776,fig.

Twórcy

autor
  • Tabriz University, Tabriz, Iran
autor

Bibliografia

  • 1. O'Toole, J. C. and Soemartono. Evaluation of a simple technique for characterizing rice root systems in relation to drought resistance. Euphytica 30 (1981) 283-290.
  • 2. Yoshida, S. and Hasegawa, S. The rice root system: its development and function. In: Drought Resistance in Crops with Emphasis on Rice IRRI, Los Banos, Philippines, 1982, 97-114.
  • 3. Sharp, R. E. and Davies W. J. Regulation of growth and development of plants growing with a restricted supply of water. In: Plants Under Stress (Jones, H. G., Flowers, T. L. and Jones M. B. Eds.), Cambridge University Press, London, 1989, 72-93.
  • 4. Hemamalini, G. S., Shashidhar, H. E. and Hittalmani, S. Molecular marker assisted tagging of morphological and physiological traits under two contrasting moisture regimes at peak vegetative stage in rice (Oryza sativa L.) Euphytica 112 (2000) 69-78.
  • 5. Ekanayake, I., O’Toole, J. C., Garrity. D. P. and Masajo, T. M. Inheritance of root characters and their relations to drought resistance in rice. Crop Sci. 25 (1985) 927-933.
  • 6. O'Toole, J. C. Breeding for drought resistance in cereals. in: Drought Resistance In Cereals (Baker, F. W. G. Ed.), CAB International Wallingford, UK, 1989, 107-116.
  • 7. Thanh, N. D., Zheng, H. G., Dong, N. V., Trinh, L. N., Ali, M. L. and Nguyen, H. T. Genetic variation in root morphology and microsatellite DNA loci in upland rice (Oryza sativa L.) from Vietnam. Euphytica 105 (1999) 43-51.
  • 8. Shashidhar, H. E., Hemamalini G. S. and Hittalmani, S. Molecular marker-assisted tagging of morphological and physiological traits at the peak vegetative stage: two contrasting moisture regimes. In: Genetic Improvement Of Rice For Water-Limited Environments (Ito, O., O'Toole, J. and Hardy, B. Eds), IRRI, Los Banos, Philippines, 1999, 239-256.
  • 9. Cao, B. and Oard, J. H. Pedigree and RAPD based DNA analysis of commercial U. S. Rice. Cultivars. Crop Sci. 37 (1997): 1630-1635.
  • 10. SAS institute, Inc. SAS language guide for personal computers. Edition 6.12, Cary, North Carolina, USA, 1996.
  • 11. Huang, N., McCouch. S. R, Mew. S., Parco. A. and Guiderdoni. E. Development of an RFLP map from a doubled haploid population in rice. Rice Genet. Newslett. 11 (1994) 134-137.
  • 12. Paterson, A. H. Genome Mapping in Plants. Academic Press, California and R. G. Landes Company, Austin, USA, 1996.
  • 13. Haley, C. S. and Anderson, L. Linkage mapping of quantitative trait loci in plants and animals. in: Genome Mapping - A Practical Approach (Dear, P. H., Ed.), Oxford University Press. Inc., New York, 1997, 49-71.
  • 14. Price, A. H., Steele, K. A., Moore, B. J., Barraclough, P. B. and Clark L. J. A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability. Theor. Appl. Genet. 100 (2000) 49-56.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-e2304e2e-2bc5-4ef1-923f-c8165862e240
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