One-step purification of vitronectin from human plasma by affinity chromatography on phage-displayed peptides

• Autorzy: Skurzynski S.
• Języki publikacji: EN

Abstrakty

EN

 A novel affinity purification method for rapid isolation of vitronectin (VN) from human plasma is described. Recently we have used phage display technology to obtain clones expressing peptides with high binding activity for VN. The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Human plasma was applied to the column and bound VN was eluted using 0.5 M acetic acid, giving purity exceeding 90 %. The developed method is a convenient alternative to conventional antibody-antigen affinity chromatography techniques for purification of VN, as it offers low ligand cost, is rapid and ensures good protein recovery from human plasma.

Słowa kluczowe

EN

affinity chromatography; human plasma; vitronectin; purification; phage display peptide

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2010
• Tom: 57
• Numer: 1
• Opis fizyczny: p.89-93,fig.,ref.

Twórcy

autor

Skurzynski S.

• Medical University of Lodz, Lodz, Poland

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