The influence of lectins isolated from pea and soybean seeds on performance and pancreatic enzyme activity in rats

• Autorzy: Czerwinski J. , Leontowicz H. , Leontowicz M. , Krzeminski R.
• Języki publikacji: EN

Abstrakty

EN

Lectin extracts were isolated from pea (PSA) and soyabean (SBA) seeds. In agglutination tests with human erythrocytes, SBA lectins showed higher activity than PSA lectins. The highest haemagglutination activity was observed with blood type AB, 50% lower activity with types A and 0, and the lowest, with type B. The experiment was performed on 30 male Wistar rats fed ad libitum a control diet or the same diet supplemented with PSA or SBA at a rate of 15 mg/day/rat for 14 days. Supplementation with lectins had a positive effect on feed intake and body weight gain, which differed significantly in the PSA group and numerically in the SBA group. PSA decreased the amylase activity in pancreatic juice and significantly decreased plasma glucose concentrations. Ca, P, Na, K, and Cl levels in pancreatic juice were similar in all groups. In rats fed the diets with lectin extracts, total pancreatic trypsin activity was significantly lower than in the control group and numerically lower in pancreatic juice. The results indicate that the effect of agglutinins from pea and soyabean seeds on pancreatic enzyme activity does not correlate with their agglutination activity.

• Wydawca: -
• Czasopismo: Journal of Animal and Feed Sciences
• Rocznik: 2006
• Tom: 15
• Numer: 4
• Opis fizyczny: p.651-659,ref.

Twórcy

autor

Czerwinski J.

• Polish Academy of Sciences, 05-110 Jablonna, Poland

autor

Leontowicz H.

autor

Leontowicz M.

autor

Krzeminski R.

Bibliografia

• Bardocz S., Grant G., Pusztai A., Ewen S.W.B., 1999. Lectins as gut growth factors - a summary. In: S. Bardocz, G. Hajós, A. Pusztai (Editors). COST 98. Effects of Antinutrients on the Nutritional Value of Legume Diets. Eur. Commun. 6, 61-64
• Czerwiński J., Leontowicz H., Leontowicz M., Gralak M.A., 2005. Response of rats to a moderate intake of soyabean lectin. J. Anim Feed Sci. 14, Suppl. 1, 537-540
• Erlanger B.F., Kokowsky N., Cohen W., 1961. The preparation and properties of two new chromogenic substrates of trypsin. Arch. Biochem. Biophys. 95, 271-278
• Gelencsér É., Hajós G., Zduńczyk Z., Jędrychowski L., 2000. Content of lectins in respect to the level of protein and other antinutritional factors in Polish pea varieties. Pol. J. Food Nutr. Sci. 9/50, 3S, 91-94
• Grant G., Alonso R., Edwards J.E., Murray S., 2000. Dietary soya beans and kidney beans stimulate secretion of cholecystokinin and pancreatic digestive enzymes in 400-day-old Hooded-Lister rats but only soya beans induce growth of the pancreas. Pancreas 20, 305-312
• Grant G., Henderson L.T., Edwards J.E., Ewan E.C., Bardocz S., Pusztai A., 1997. Kidney bean and soybean lectins cause enzyme secretion by pancreatic acini in vitro. Life Sci. 60, 1589-1595
• Grant G., More L.J., Mc Kenzie N.H., Dorward P.M., Buchan W.C., Telek L., Pusztai A., 1995.
• Nutritional and haemagglutination properties of several tropical seeds. J. Agr. Sci. 124, 437-445
• Herzig K.H., Bardocz S., Grant G., Nustede R., Folsch U.R., Pusztai A., 1997. Red kidney bean lectin is a potent cholecystokinin releasing stimulus in the rat inducing pancreatic growth. Gut 41, 333-338
• Jordinson M., Deprez P.H., Playford R.J., Heal S., Freeman T.C., Alison M., Calam J., 1996. Soybean lectin stimulates pancreatic exocrine secretion via CCK-A receptors in rats. Amer. J. Physiol. 270, G653-G659
• Kelsall A., Fitzgerald A.J., Howard C.V., Evans R.C., Singh R., Rhodes J.M., Goodlad R.A., 2002. Dietary lectins can stimulate pancreatic growth in the rat. Int. J. Exp. Pathol. 83, 203-208
• Koninkx J.F.J.G., 1995. Enterocyte-like Caco-2 cells as a tool to study lectin interaction. In: A. Pusztai, S. Bardocz (Editors). Lectins Biomedical Perspectives. Taylor and Francis Ltd., London, pp. 81-101
• Leontowicz H., Leontowicz M., Biernat M., Gralak M., Krzemiński R., Czerwiński J., 2000. The effect of pea lectins on pancreas and jejunum in rats fed semipurified diet. In: S.G. Pierzynowski, J.M. Gee, J. Svendsen (Editors). COST 98. Effects of Antinutrients on the Nutritional Value of Legume Diets. Eur. Commun. 9, 111-115
• Leontowicz H., Leontowicz M., Czerwiński J., Kostyra H., Krzemiński R., 2004. Influence of lectins isolated from soyabean seeds and evening primrose seeds and cake on rat metabolism. 4th International Workshop on Antinutritional Factors in Legume Seeds and Oilseeds. EAAP Publication No.110, Toledo (Spain), pp. 115-119
• Li Z., Li D., Qiao S., 2003a. Effects of soybean agglutinin on nitrogen metabolism and on characteristics of intestinal tissues and pancreas in rats. Arch. Tierernähr. 57, 369-380
• Li Z., Li D., Qiao S., Zhu X., Huang C., 2003b. Anti-nutritional effects of a moderate dose of soybean agglutinin in the rat. Arch. Tierernähr. 57, 267-277
• Liener I.E., 1989. Control of antinutritional and toxic factors in oil seeds and legumes. In: W.E.W. Lusas, D.R. Erikson, W. Nip (Editors). Food Uses of Whole Oil and Protein Seeds. Amer. Oil Chem. Soc., pp. 344-371
• Liener I.E., 1994. Implication of antinutritional components in soybean foods. Crit. Rev. Food Sci. Nutr. 34, 31-67
• Lowry O.H., Rosebrough A.L., Farr A.J., Randal R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275
• Pusztai A., 2000. Biological effects of plant lectins on the gut: Metabolic consequences and applications. In: S.G. Pierzynowski, J.M. Gee, J. Svendsen (Editors). COST 98. Effects of Antinutrients on the Nutritional Value of Legume Diets. Eur. Commun. 9, 141-142
• Pusztai A., Bardocz S., 1999. Lectins in health and therapy. In: S. Bardocz, G. Hajós, A. Pusztai (Editors). COST 98. Effects of Antinutrients on the Nutritional Value of Legume Diets. Eur. Commun. 6, 1-13
• Rådberg K., Biernat M., Linderoth A., Zabielski R., Pierzynowski S.G., Weström B.R., 2001. Enteral exposure to crude red kidney bean lectin induces maturation of the gut in suckling pigs. J. Anim. Sci. 79, 2669-2678
• Santiago J.G., Levy-Benshimol A., Carmona A., 1993. Effect of Phaseolus vulgaris on glucose absorption, transport and metabolism in rat everted intestinal sacs. J. Nutr. Biochem. 4, 426-430
• Schulze H., Saini H.S., Huisman J., Hessing M., Berg W.V.D., Verstegen W.A., 1995. Increased nitrogen secretion by inclusion of soya lectin in the diets of pigs. J. Sci. Food Agr. 69, 501-510