Localization of nitrogenase and nitrate reductase in the Cycas-Anabaena cycadeae association

• Autorzy: Sharma A , Mishra P. , Kumar A.
• Języki publikacji: EN

Abstrakty

EN

Induction of nitrate reductase (NR) activity in coralloid roots of Cycas revoluta was observed after 8 h incubation in 0.02 M KNO3. Other plants growing near Cycas showed a higher level of NR immediately when incubated in KNO3. In contrast to NR, intact coralloid roots showed very high nitrogenase activity (~1.2 to 1.6 µmol C2H4 g fresh wt-1 h-1) under both light and dark conditions as compared to transverse sections of roots. Localization of NR and nitrogenase was tested in coralloid roots using different sets of roots and also in the endophyte. Our results showed that NR activity was mainly due to the endophyte (Anabaena cycadeae); coralloid roots lacked it, as no NR activity was observed in chloramphenicol-treated intact root samples.

Słowa kluczowe

EN

localization; nitrogenase; nitrate reductase; Cycas-Anabaena cycadeae association; nitrogenase activity; coralloid root; Cycas revoluta; root sample

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2004
• Tom: 46
• Opis fizyczny: p.213-216,fig.,ref.

Twórcy

autor

Sharma A

• Pant University of Agriculture and Technology, Pantnagar, 263 145, Udham Singh Nagar, Uttar Pradesh, India

autor

Mishra P.

autor

Kumar A.

Bibliografia

• Allen MB, and Arnon DI. 1955. Studies on nitrogen-fixing blue green algae. I Growth and nitrogen fixation by Anabaena cylindrica. Plant Physiology 30: 366-372.
• Camm EL, and Stein JR. 1974. Some aspects of the nitrogen metabolism of Nodularia spumigena. Canadian Journal of Botany 52: 719-726.
• Costa JL, Paulsrud P, and Lindblad P. 1999. Cyanobiont diversity within coralloid roots of selected cycad species. FEMS Microbiology and Ecology 28: 85-91.
• Lindblad P. 2002. Cyanobacteria in symbiosis with cycas In: Rai AN, Bergman B, and Rasmussen U [eds.], Cyanobacteria in symbiosis, 195-205. Kluwer Academic Publ., The Netherlands.
• Lindblad P, and Bergman B. 1986. Glutamine synthetase: activity and localization in cyanobacteria of cycads Cycas revoluta and Zamia skinneria Planta 169: 1-7.
• Lowry RH, and Evans HJ. 1964. Preparation and some properties of a soluble nitrate reductase from Rhizobium japonicum. Biochemistry and Biophysics Acta 85: 377-389.
• Mackinney G. 1941. Absorption of light by chlorophyll solution. Journal of Biological Chemistry 140: 315-322.
• Meeks JC, Enderlin CS, Joseph CM, Chapman JS, and Lollar MWL. 1985. Fixation of 13N2 and transfer of fixed nitrogen in the Anthoceros-Nostoc symbiotic association. Planta 164: 406-414.
• Peters GA, and Meeks JC. 1989. The Azolla-Anabaena symbiosis: Basic biology. Plant Molecular Biology 40: 193-210.
• Rai AN, Rowell P, and Stewart WDP. 1983. NH4+ assimilation and nitrogenase regulation in the lichen Peltigera aphthosa wild. Archives of Microbiology 134: 136-142.
• Rai AN, Lindblad P, and Bergman B. 1986. Absence of the glutamine synthetase linked methyl ammonium (ammonium) transport system in the cyanobiont of Cycas cyanobacterial symbiosis. Planta 169: 379-381.
• Sharma A, Srivastava M, Rathore DS, Srinivas G, and Kumar A. 1988. Sources of reductant supply for N2 fixation in Cycas Anabaena cycadeae association. In: Proceedings of National Symposium. Cyanobacterial nitrogen fixation, 63-70. Cuttak.
• Sik T, and Barabas I. 1977. The correlation of nitrate reductase and nitrogenase in Rhizobium symbiosis. In: Newton W, Postgate JR, and Rodríguez-Barrueco C [eds.], Proceedings "Recent development in nitrogen fixation", 366-373. Academic Press, London.
• Srivastava M, Sharma A, and Kumar A. 1988. Isolation and characterization of the nitrogen-fixing cyanobacteria A. azollae. In: Skinner FA [ed.], Nitrogen fixation with nonlegume, 77-82. Kluwer Academic Publ., The Netherlands.
• Stewart WDP, Fitzgerald GP, and Burris RH. 1967. In situ studies on N2 fixation using acetylene reduction technique. Proceedings of the National Academy of Sciences, U.S.A. 58: 2071-2078.
• Stewart WDP. 1980. Some aspect of structure and function in nitrogen-fixing cyanobacteria. Annual Review in Microbiology 34: 497-536.
• Stewart WDP, Rowell P, and Rai AN. 1983. Cyanobacteria eukaryotic plant symbiosis. Annul de Microbiology 134: 205-228 .