Development of a rapid, reliable and simple multiplex PCR assay for early detection of transgenic plant material

• Autorzy: Xu C J , Yang L. , Chen K.S.
• Języki publikacji: EN

Abstrakty

EN

Multiplex PCR is a variant of conventional PCR which includes two or more pairs of primers in a single reaction to amplify corresponding genes simultaneously. In this study, a reliable multiplex PCR analysis protocol was established for simple and fast detection of transgenes in plant materials. Two pairs of primers, corresponding to neomycin phosphotransferase gene and 1-aminocyclopropane-1-carboxylate synthase gene, were selected for target and resident gene respectively. The method bypasses routine DNA extraction, requires only very little amount of plant tissue and produces reliable results as shown by successful discrimination of transformed and non transformed tobacco, tomato and kumquat materials. The method facilitates early identification of transgenic buds when they are still quite small.

Słowa kluczowe

EN

genetic transformation; transgenic plant; plant genetics; DNA extraction; plant tissue; polymerase chain reaction; plant material

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2005
• Tom: 27
• Numer: 3A
• Opis fizyczny: p.283-288,fig.,ref.

Twórcy

autor

Xu C J

• Zhejiang University, Hangzhou 310029, P.R.China

autor

Yang L.

autor

Chen K.S.

Bibliografia

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