An efficient method of genomic DNA isolation from plant tissues

• Autorzy: Strozycki P. M. , Legocki A.B.
• Języki publikacji: EN

Abstrakty

EN

The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.

Słowa kluczowe

EN

Lupinus luteus; DNA isolation; plant tissue; genome

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 3
• Opis fizyczny: p.329-331,fig

Twórcy

autor

Strozycki P. M.

• Polish Academy of Sciences, Z.Noskowskiego 12-14, 32-607 Poznan, Poland

autor

Legocki A.B.

Bibliografia

• 1. Petit,A., Stougaard,J., Kuehle, A., Marcker, K.A. & Tempe, J. (1987) Transformation and regene­ration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixa­tion. Mol. Gen. Genet. 207,245-250.
• 2. Trieu-Cuot, P. & Courvalin, P. (1983) Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminogIycoside phos­photransferase type III. Gene 23,331-341.
• 3. Hamill, J.D., Rounsley, S., Spencer, A., Todd, G. & Rhodes, M.J.C. (1991) The use of polymerase chain reaction in plant transformation studies. Plant Cell Reports 10,221-224.
• 4. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.