Heterogeneity of Borrelia burgdorferi sensu lato and their reflection on immune response

• Autorzy: Stefancikova A. , Derdakova M. , Stepanova G. , Pet'ko B. , Szestakova E. , Skardova I. , Cislakova L.
• Języki publikacji: EN

Abstrakty

EN

Geographically different strains of Borrelia burgdorferi sensu lato (B. burgdorferi sensu stricto Ir 105, B. burgdorferi s.s. + B. afzelii V 123, B. garinii Ir 112 - isolates from eastern Slovakia, B. garinii K24 - isolate from western Slovakia and B. burgdorferi s.s. B 31 - American strain) were compared as antigens for serological study of Lyme borreliosis by IgG ELISA on a group of horses from eastern Slovakia. In a set of 101 horse serum samples, positivity with the use of Ir 105 strain was 53 (52.4%), with V 123 51 (51.49%), with Ir 112 48 (47.5%), with K 24 47 (46.5%) and with B 31 only 25 (24.7%). The seroprevalence between strains B 31 and Ir 105, B 31 and V 123, B31 and Ir112, B 31 and K 24 differed statistically significantly ( test chi2, p<0.05); however, the differences between strains Ir 105, V 123, Ir 112 and K 24 were insignificant. Consistency of positive and negative findings between American and Slovak strains ranged from 50.5-62.4%. Comparison of Slovak strains (Ir 105, V 123, Ir 112 and K 24) consistency of positive and negative findings was higher from 79.2-95.04% The highest consistency of findings was reached comparing strains Ir 112 and K 24, and the same high agreement of results was observed between the strains Ir 105 and V 123 and also Ir 112 and Ir 105. Higher consistency of findings of serologically examined horses with geographically close strains is in accordance with greater similarity of protein profiles of Slovak strains compared to the American strain.

Słowa kluczowe

EN

Borrelia burgdorferi; heterogeneity; immune response; ELISA test; horse; seroprevalence; immunoglobulin; Borrelia afzelii; Borrelia garinii; farm animal; animal disease; diagnosis; cross reaction

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2005
• Tom: 12
• Numer: 2
• Opis fizyczny: p.211-216,fig.,ref.

Twórcy

autor

Stefancikova A.

• Slovak Academy of Sciences, Hlinkova 3, 040 01 Kosice, Slovak Republic

autor

Derdakova M.

autor

Stepanova G.

autor

Pet'ko B.

autor

Szestakova E.

autor

Skardova I.

autor

Cislakova L.

Bibliografia

• 1. Anderson JF, Magnarelli LA: Avian and mammalian hosts for spirochete-infected ticks and insects in a Lyme disease focus in Connecticut. Yal J Biol Med 1984, 57, 627-641.
• 2. Anderson JF: Epizootiology of Lyme borreliosis. Scand J Infect Dis Supplement 1991, 77, 23-24.
• 3. Appel MJG: Lyme disease in dogs and cats. Compend Cont Educ Pract Vet 1990,12, 617-626.
• 4. Bergström S, Sjöstedt A, Dotevall L, Kaijser В, Ekstrand- Hammarström E, Wallberg C, Skogman G, Barbour AG: Diagnosis of Lyme borreliosis by an enzyme immunoassay detecting immunoglobulin G reactivve to purified Borrelia burgdorferi cell components. Eur J Clin Microbiol Infect Dis 1991,10, 422-427.
• 5. Bunikis J, Olsén В, Westman G, Bergström S: Variable serum immunoglobulin responses against different Borrelia burgdorferi sensu lato species in a population at risk for and patients with Lyme disease. J Clin Microbiol 1995, 33, 1473-1478.
• 6. Burgess EC, Patrican LA: Oral infection of Peromyscus maniculatus with Borrelia burgdorferi and subsequent transmission by Ixodes damini. Am J Trop Med Hyg 1987, 36, 402-407.
• 7. Cutler SJ, Wright DJM, Luckhurst VH: Simplified method for the interpretation of immunoblots for Lyme borreliosis. Immun Med Microbiol 1993, 6, 281-286.
• 8. Derdáková M, Beati L, Peťko B, Stanko M, Fish D: Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-Single-Standard Conformation Polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from Czech Republic. Appl Environ Microbiol 2003, 69, 509-516.
• 9. Dorwood DW, Schwan TG, Garon CF: Immune capture and detection of Borrelia burgdorferi antigens in urine, blood or tissues from infected ticks, mice, dogs and humans. J Clin Microbiol 1991, 31, 1388- 1391.
• 10. Eng TR, Wilson ML, Spielman A, Lastovica CC: Greater risk of Borrelia burgdorferi infection in dogs than in people. J Infect Dis 1988, 158, 1410-1411.
• 11. Gern L, Chang Min Hu, Kociánová E, Vyrosteková V, Øeháèek J: Genetic diversity of Borrelia burgdorferi sensu lato isolates obtained from Ixodes ricinus ticks collected in Slovakia. Eur J Epidemiol 1999, 15, 665-669.
• 12. Goodman JL, Jurkovich P, Kramber JM Johnson RC: Molecular detection of persistent Borrelia burgdorferi in urine of patients with active Lyme disease. Infect Immunol 1991, 59, 269-270.
• 13. Hanincová K, Schäfer SM, Etti S, Sewell HS, Taragelová V, Žiak D, Labuda M, Kurtenbach: Association of B. afzelii with rodents in Europe. Parasitology 2003, 126, 11-20.
• 14. Hansen K, Bangsborg JM, Fjordvang H, Pedersen NS, Hindersson P: Immunochemical characterization and isolation of the gene for a Borrelia burgdorferi immunodominant 60-kilodalton antigen common to a wide range of bacteria. Infect Immun 1988, 56, 2047-2053.
• 15. Hansen K, Pii K, Lebech AM: Improved immunoglobulin M serodiagnosis in Lyme borreliosis by using a ^-capture enzyme-linked immunosorbent assay with biotinylated Borrelia burgdorferi flagela. J Clin Microbiol 1991, 29, 166-173.
• 16. Hauser U, Lehnert G, Lobentanzer R, Wilske В: Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato. J Clin Microbiol 1997, 35, 1433-1444.
• 17. Hauser U, Lehnert G, Wilske B: Diagnostic value of proteins of three Borrelia species (Borrelia burgdorferi sensu lato) and implications for development and use of recombinant antigens for serodiagnosis of Lyme borreliosis in Europe. Clin Diag Lab Immunol 1998, 5, 456-462.
• 18. Isogai E, Isogai H, Masuzawa T, Yanagihara Y, Sato N, Hayash S, Maki T, Moci M: Serological survey for Lyme disease in sika deer (Cervus nippon yesoensis) by enzyme-linked immunosorbent assay (ELISA). Microbiol Immunol 1991, 35, 695-703.
• 19. Isogai H, Isogai E, Masuzawa T, Yanagihara Y, Matsubara M, Shimanuki M, Seta T, Fukai K, Kurosawa N, Enokidani M, Nakamura T, Tajima M, Takahashi K, Fujíí N: Seroepidemiological survey for antibody to Borrelia burgdorferi in cows. Microbiol Immunol 1992, 36, 1023-1030.
• 20. Johnson RC, Schmidt GP, Hyde FW, Steigerwalt AG, Berenner DJ: Borrelia burgdorferi sp. nov.: etiological agent of Lyme disease. Int J System Bacteriol 1984, 34, 496-497.
• 21. Kmety E, Bakoss P: To the issue of the selection of antigens for themicroscopic agglutination reaction in leptospirosis. Čs Epidem 1978, 27, 247-252.
• 22. Kmety E, Řeháček J, Vyrosteková V: Investigations of ticks for the presence of Borrelia in Czechoslovakia. Zentrabl Bakteriol Hyg A 1986, 263, 468-470.
• 23. Kmety E, Řeháček J, Vyrosteková V, Kociánová E, Guričová D: Infestation of ticks with Borrelia burgdorferi and Francisella tularensis in Slovakia. Bratisl Lek Listy, 1990, 91, 251-266.
• 24. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970, 227, 680-685.
• 25. Lowry OH, Rosebrough NS, Farr AL, Randall RG: Protein measurement with the Folin phenol reagent. J Biol Chem 1951, 193, 265-275.
• 26. Luft BJ, Jiang W, Munoz P, Dattwyler RJ, Gorevic PD: Biochemical and immunological characterization of the surface proteins of Borrelia burgdorferi. Infect Immun 1989, 57, 3637-3645.
• 27. Macuzawa T, Takada N, Kudeken M, Fukui T, Yano Y, Ishiguro F, Kawamura Y, Imai Y, Ezaki T: Borrelia sinica sp. nov., a Lyme disease-related Borrelia species isolated in China. Int J Syst Evol Microbiol 2001,51, 1817-1824.
• 28. Madigan JE: Lyme disease (Lyme borreliosis) in horses. Equine Pract 1993, 9, 429-434.
• 29. Magnarelli LA, Anderson JF, Johnson RC, Nadelman RB, Wormser GP: Comparison of different strains of Borrelia burgdorferi sensu lato used as antigen in enzyme-linked immunosorbent assay. J Clin Microbiol 1994, 32, 1154-1158.
• 30. Magnarelli LA, Anderson JF, Johnson RC: Analyses of mammalian sera in enzyme-linked immunosorbent assay with different strains of Borrelia burgdorferi sensu lato. J Wild Dis 1995, 31, 159-165.
• 31. Margolis CZ: Used of clinical algorithms. JAMA 1983,249,627-632.
• 32. Mateička F, Kozáková D, Rosa PA, Kmety E: Genotype identification of Slovak Borreliae isolates by PCR method. Proceedings of the Slovak Borrelia Days (SBD) 95, October 25-26, 1995, 5, Košice.
• 33. Mateička F, Kozáková D, Rosa PA, Kmety E: Identification of Borrelia burgdorferi sensu lato Tick isolates from Slovakia by PCR Typing with 16S rRNA Primers. Zbl Bakteriol 1997, 286, 355-361.
• 34. Norman GL, Antig JM, Bigaignon G, Hogrefe WR: Serodiagnosis of Lyme borreliosis by Borrelia burgdorferi sensu stricto, B. garinii, and B. afzelii western blots (immunoblots). J Clin Microbiol 1996, 34(7), 1732-1738.
• 35. Parker JL, White KK: Lyme boreliosis in cattle and horses: A review of the literature. Co Vet 1992, 82, 253-274.
• 36. Peťko B, Štefančíková A, Tresová G, Peterková J, Škardová I, Prokopčáková H, Čisláková L: Common tick (Ixodes ricinus) as a source of infection of humans and dogs with the agent of Lyme borreliosis in Slovakia. Slov Vet Čas 1996, 21, 313-318.
• 37. Reisenauer R: Mathematico-statistical methods and their applications in technology. SNTL, Praha 1970 (In Czech).
• 38. Russell H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B: Enzyme-linked immunosorbent assay and indirect immunofluorescence assay for Lyme disease. J Infect Dis 1984, 149, 465-470.
• 39. Simpson WJ, Schrumf ME, Schwan TG: Reactivity of human Lyme borreliosis sera with 39-kilodalton antigen specific to Borrelia burgdorferi. J Clin Microbiol 1990, 28, 1329-1337.
• 40. Štefančíková A, Tresová G, Peťko B, Škardová І, Sesztáková E: ELISA comparison of three whole-cell antigens of Borrelia burgdorferi sensu lato in serological study of dogs from area of Košice, Eastern Slovakia. Ann Agric Environ Med 1998, 5, 25-30.
• 41. Štepánová-Tresová G, Peťko B, Štefančíková A, Nadzamová D: Occurrence of Borrelia burgdorferi sensu stricto, Borrelia garini and Borrelia afzelii in the Ixodes ricinus ticks from Eastern Slovakia. Eur J Epidemiol 2000, 16, 105-109.
• 42. Towbin H, Staehlin T, Gordon J: Electroforetic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci USA 1979, 76, 4350-4354.
• 43. Tresová G, Mateička T, Peťko B, Kozárová D: Preliminary identification of Borrelia burgdorferi isolates from ticks Ixodes ricinus in eastern Slovakia. Epidemiol Mikrobiol Imunol 1997, 46, 36-38.
• 44. Wang G, van Dam AP, Schwartz I, Dankert J: Molecular typing of Borrelia burgdorferi sensu lato: taxonomie, epidemiological and clinical implications. Clin Microbiol Rev 1999, 12, 633-653.
• 45. Wilske B, Preac-Mursic V, Schierz G, Kuhbeck R, Barbour AG, Kramer M: Antigenic variability of Borrelia burgdorferi. Ann NY Acad Sci 1988, 539, 126-143.
• 46. Wilske B, Preac-Mursic V, Göbel UB, Graf B, Jauris-Heipke S, Soutschek E, Schwab E, Zumstein G: An Osp A serotyping system for Borrelia burgdorferi based on reactivity with monoclonal antibodies and OspA sequence analysis. J Clin Microbiol 1993, 31, 340-350.
• 47. Wilske B, Preac-Mursic V, Jauris S, Hofmann A, Pradel I, Soutschek E, Schwab E, Will G, Wanner G: Immunological and molecular polymorphisms of OspC, an immunodominant major outer surface protein of Borrelia burgdorferi. Infect Immun 1993, 61, 2182- 2191.
• 48. Wilske B, Schierz G, Preac-Mursic V, Weber K, Pfister HW, Einhäupl K: Serological diagnosis of erythrema migrans disease and related disorders. Infection 1984, 12, 331-337.