Differentiation of European brown hare syndrome and rabbit haemorrhagic disease viruses by amplification and restriction analysis of capsid protein gene fragment

• Autorzy: Chrobocinska M
• Języki publikacji: EN

Abstrakty

EN

The aim of the studies was differentiation of EBHS and RHD viruses by amplification of the fragment coding the first part of the capsid protein (VP60) and digestion of this fragment by restriction endonucleases. The fragments of 4 strains of EBHS (two Polish isolated in 1992 and 1998; two standard Italian and French) and 5 strains of RHD (Polish strains isolated in 1988, 1994, 1997 and 1998) viruses were amplified by RT-PCR with the use of primers selected for each virus. The specific fragments of 726 bp of EBHS and 734 bp of RHD viruses were demonstrated after amplification with the use of primers selected for each virus. Negative results were obtained when primers selected for the RHD virus were used to amplify the fragment of EBHS virus and on the contrary. The hybridisation by the Southern blot method revealed the cross reaction of DIG-Iabelled probes prepared for each virus. The endonucleases BamH I and Pst I were used in the restriction analysis of fragments. The 734 bp fragments of all strains of RHD virus digested by BamH I and Pst I enzymes demonstrated the same restriction profiles. The 726 bp fragments of strains of EBHS virus were not digested by Pst I, whereas in two strains (standard Italian and Polish isolated in 1988) after digestion with BamH I different restriction profiles in comparison with RHD virus were demonstrated.

Słowa kluczowe

EN

restriction analysis; European brown hare syndrome; capsid protein gene; polymerase chain reaction; rabbit hemorrhagic disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2000
• Tom: 44
• Numer: 2
• Opis fizyczny: p.111-123,fig.

Twórcy

autor

Chrobocinska M

• National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Asgari S., Hardy J.R.E., Cooke B.D.: Sequence analysis of rabbit haemorrhagic disease virus (RHDV) in Australia: alterations after its release. Arch. Virol. 1999, 144, 135-145.
• 2. Boga J.A., Marin M.S., Casais R., Prieto M., Parra F.: In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV). Virus Res. 1992, 26, 33-40.
• 3. Capucci L., Scicluna M.T., Lavazza A.: Diagnosis of viral haemorrhagic disease of rabbits and the European brown hare syndrome. Rev. Sci. Tech. Off. Int. Epiz. 1991, 10, 347-370.
• 4. Capucci L., Frigoli G., Ronshold L., Lavazza A., Brocchi E., Rossi C.: Antigenicity of the rabbit hemorrhagic disease virus studied by its reactivity with monoclonal antibodies. Virus Res. 1995, 37, 221-238.
• 5. Capucci L., Chasey D., Lavazza A., Westcott D.: Preliminary characterization of a non-haemagglutinating strain of rabbit haemorrhagic disease virus from the United Kingdom. J. Vet. Med. B 1996, 43, 245-250.
• 6. Capucci L., Fusi P., Lavazza A., Pacciarini M., Rossi C.: Detection and preliminary characterization of a new rabbit calicivirus related to rabbit haemorrhagic disease virus but nonpathogenic J. Virol. 1996, 70, 8614-8623.
• 7. Capucci L., Fallacara F., Grazioli S., Lavazza A., Pacciarini M.L., Brocchi E.: A further step in the evolution of rabbit haemorrhagic disease virus: the appearance of the first consistent antigenic variant. Virus Res. 1998, 58, 115-126.
• 8. Chrobocińska M., Górski J.: Prevalence of infection with EBHS (European brown hare syndrome) virus in hares in Poland. Bull. Vet. Inst. Pulawy 1995, 39, 17-21.
• 9. Chrobocińska M.: Rabbit haemorrhagic disease virus: studies on field strains isolated in Poland. Bull. Vet. Inst. Pulawy 1996, 40, 61-67.
• 10. Chrobocińska M.: ELISA and Western blot tests used for the diagnosis of infection caused by EBHS virus in hares. Medycyna Wet. 1997, 53, 343-345.
• 11. Chrobocińska M.: Amplification of capsid gene of European brown hare syndrome virus by reverse transcriptase-polymerase chain reaction. Bull. Vet. Inst. Pulawy 1998, 42, 125-133.
• 12. Chrobocińska M., Kozaczyński W., Mizak Z., Mizak B.: The case of infection of hare with European brown hares syndrome virus. Medycyna Wet. 1999, 55, 750-752.
• 13. Fitzner A., Niedbalski W.: Application of ELISA for the virologic and serologic diagnosis of viral haemorrhagic disease of rabbit (VHD). Bull. Vet. Inst. Pulawy 1996, 40, 69-76
• 14. Fitzner A., Kęsy A., Niedbalski W., Paprocka G.: Application of RT-PCR for identification of viral haemorrhagic disease virus (RHDV) of rabbits isolated in Poland. Medycyna Wet. 1999, 55, 120-122.
• 15. Fitzner A., Kęsy A., Niedbalski W., Paprocka G.: Detection of rabbit haemorrhagic disease virus (RHDV) in sick animals. Medycyna Wet. 2000, 56, 43-48.
• 16. Le Gall G., Huguet S., Vende P., Vautherot J.F., Rasschaert D.: European brown hare syndrome virus: molecular cloning and sequencing of the genome. J. Gen. Virol. 1996, 77, 1693-1697.
• 17. Le Gall G., Arnauld C., Boilletot E., Morisse J.P., Rasschaert D.: Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988 to 1995. J. Gen. Virol. 1998, 79, 11-16.
• 18. Gould A.R., Kattenbelt J.A., Lenghaus C., Morrissy C., Chamberlain T., Collins B.J., Westbury H.A.: The complete nucleotide sequence of rabbit haemorrhagic disease virus (Czech strain V351): use of polymerase chain reaction to detect replication in Australia vertebrates and analysis of viral population sequence variation. Virus Res. 1997, 47, 7-17.
• 19. Guittre S., Bagiński I., Le Gall G., Trepo C., Cova L.: Detection of rabbit haemorrhagic disease virus isolated and sequence comparison of the N-terminus of the capsid protein gene by the polymerase chain reaction. Res. Vet. Sci. 1995, 58, 128-132.
• 20. Kęsy A., Fitzner A., Niedbalski W., Paprocka G., Walkowiak B.: A new variant of the viral haemorrhagic disease of rabbits virus. Res. Sci. Tech. Off. Int. Epiz. 1996, 15, 1029- 1035.
• 21. Laurent S., Vautherot J-F., Le Gall G., Madelaine M.-F., Rasschaert D.: Structural, antigenic and immunogenic relationships between European brown hare syndrome virus and rabbit haemorrhagic disease virus. J. Gen. Virol. 1997, 78, 2803-2811.
• 22. Meyers G., Wirblich C., Thiel H.J.: Rabbit haemorrhagic disease virus – molecular cloning and nucleotide sequencing of a calicivirus genome. Virology 1991, 184, 664-676.
• 23. Nardelli S., Agnoletti F., Costantini F., Parpajola R.: Diagnosis of rabbit haemorrhagic disease (RHD) and European brown hare syndrome (EBHS) by indirect sandwich polyclonal ELISA. J. Vet. Med. B 1996, 43, 393-400.
• 24. Neill J.D.: Nucleotide sequence of the capsid protein gene of two serotypes of San Miguel sea lion virus: ldentification of conserved and non-conserved amino acid sequences among calicivirus capsid protein. Virus Res. 1992, 24, 211-222.
• 25. Novotny N., Ros Bascunana C., Ballagi-Pordany A., Gavier-Widen D., Uhlen M., Belak S.: Phylogenetic analysis of rabbit haemorrhagic disease and European brown hare syndrome viruses by comparison of sequences from the capsid protein gene. Arch. Virol. 1997, 142, 657-673.
• 26. Parra F., Boga J.A., Marin M.S., Casais R.: The amino terminal sequence of VP60 from rabbit haemorrhagic disease virus supports its putative subgenomic origin. Virus Res. 1993, 27, 219-228.
• 27. Rasschaert D., Huguet S., Madeleine M.F., Vautherot J.F.: Sequence and genomic organization of a rabbit hemorrhagic disease virus isolated from a wild rabbit. Virus Genes 1994, 9, 121-132.
• 28. Ros Bascuana C., Novotny N., Belak S.: Detection and differentiation of rabbit hemorrhagic disease and European brown hare syndrome viruses by amplification of VP60 genomic sequences from fresh and fixed tissue specimens. J. Clin. Microbiol. 1997, 35, 2492-2495.
• 29. Schirrmeier H., Reimann I., Köllner B., Granzow H.: Pathogenic, antigenic and molecular properties of rabbit haemorrhagic disease virus (RHDV) isolated from vaccinated rabbits: detection and characterization of antigenic variants. Arch. Virol. 1999, 144, 719-735.
• 30. Wirblich C., Meyers G., Ohlinger V.F., Capucci L., Eskens U., Haas B., Thiel H.J.: European brown hare syndrome virus: relationship to rabbit haemorrhagic disease virus and other caliciviruses. J. Virol. 1994, 68, 5164-5173