Transcription factors as targets of the anti-inflammatory treatment. A cell culture study with extracts from some Mediterranean diet plants

• Autorzy: Stalinska K. , Guzdek A. , Rokicki M. , Koj A.
• Języki publikacji: EN

Abstrakty

EN

During the inflammatory response at least 2 transcription factors, NF-kB and AP-1, are involved in the altered profile of gene expression. We used human hepatoma (HepG2) and human umbilical vein endothelial cells (HUVEC) as a model system: NF-kB and AP-1 were activated by the proinflammatory cytokine IL-1 in the absence or presence of 21 selected plant extracts and the effect was evaluated by the electrophoretic mobility shift assay (EMSA). In both types of cells activation of NFkB by IL-1 was significantly inhibited by extracts from Scandix australis and Artemisia alba, whereas extracts from Amaranthus sp., Eryngium campestre, Thymus pulegioides and Reichardia picroides elicited cell-type dependent response. The IL-1-induced AP-1 activation was diminished by extracts from Scandix australis, Amaranthus sp. and Artemisia alba more potently in HUVEC, while extracts from Urospermum picroides and Scandix pecten-veneris in HepG2 cells. Inhibitory activities of plant extracts towards cytokine activated NF-kB and AP-1 depend to some extent on the order of addition of IL-1 and plant extract to the cell culture, but the mechanism of action of extract components is not clear: although plant polyphenols may participate they are unlikely to be the only mediators, and MAP kinases were found generally not involved in down-regulation of transcription factors activity by plant extracts.

Słowa kluczowe

EN

hepatoma; gene expression; Mediterranean diet; plant extract; nuclear factor-kappaB; cell culture; transcription factor; inflammation; human umbilical vein endothelial cell

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2005
• Tom: 56
• Numer: 1
• Opis fizyczny: p.157-169,fig.,ref.

Twórcy

autor

Stalinska K.

• Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland

autor

Guzdek A.

autor

Rokicki M.

autor

Koj A.