In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographis paniculata extract and andrographolide

• Autorzy: Subramanian R. , Asmawi M.Z. , Sadikun A.
• Języki publikacji: EN

Abstrakty

EN

There has been an enormous interest in the development of alternative medicines for type 2 diabetes, specifically screening for phytochemicals with the ability to delay or prevent glucose absorption. The goal of the present study was to provide in vitroevidence for potential inhibition of α-glucosidase and α-amylase enzymes, followed by a confirmatory in vivostudy on rats to generate a stronger biochemical rationale for further studies on the ethanolic extract of Andrographis paniculata and andrographolide. The extract showed appreciable α-glucosidase inhibitory effect in a concentration-dependent manner (IC50=17.2±0.15 mg/ml) and a weak α-amylase inhibitory activity (IC50=50.9±0.17 mg/ml). Andrographolide demonstrated a similar (IC50=11.0±0.28 mg/ml) α-glucosidase and α-amylase inhibitory activity (IC50=11.3±0.29 mg/ml). The positive in vitroenzyme inhibition tests paved way for confirmatory in vivostudies. The in vivostudies demonstrated that A. Paniculata extract significantly (P<0.05) reduced peak blood glucose and area under curve in diabetic rats when challenged with oral administration of starch and sucrose. Further, andrographolide also caused a significant (P<0.05) reduction in peak blood glucose and area under the curve in diabetic rats. Hence α-glucosidase inhibition may possibly be one of the mechanisms for the A. paniculataextract to exert antidiabetic activity and indicates that AP extract can be considered as a potential candidate for the management of type 2 diabetes mellitus.

Słowa kluczowe

PL

enzyme; alpha-glucosidase; alpha-amylase; inhibitory effect; Andrographis paniculata; andrographolide; peak blood glucose; postprandial hyperglycemia

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 2
• Opis fizyczny: p.391-398,fig.,ref.

Twórcy

autor

Subramanian R.

• School of Pharmacy, Universiti Sains Malaysia, Penang, Malaysia

autor

Asmawi M.Z.

autor

Sadikun A.

Bibliografia

• Akowuah GA, Zhari I, Norhayati I, Mariam A (2006) HPLC and HPTLC densitometric determination of andrographolides and antioxidant potential of Andrographis paniculata. J Food Compost Anal 19:118-126.
• Ali H, Houghton PJ, Soumyanath A (2006) α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. J Ethnopharmacol 107:449-455.
• Amroyan E, Gabrielian E, Panossian A, Wikman G, Wagner H (1999) Inhibitory effect of andrographolide from Andrographis paniculata on PAF-induced platelet aggregation. Phytomedicine 6:27-31.
• Anis E, Anis I, Ahmed S, Mustafa G, Malik A, Afza N, Hai SMA, Shahzad-ul-hussan S, Choudhary MI (2002) α Glucosidase inhibitory constituents from Cuscuta reflexa. Chem Pharm Bull 50:112-114.
• Apostolidis E, Kwon YII, Shetty K (2006) Potential of cranberry-based herbal synergies for diabetes and hypertension management. Asia Pac J Clin Nutr 15:433-441.
• Apostolidis E, Kwon YII, Shetty K (2007) Inhibitory potential of herb, fruit, and fungal-enriched cheese against key enzymes linked to type 2 diabetes and hypertension. Inn Food Sci Emer Technol 8:46-54.
• Burgos RA, Caballero EE, Sfinchez NS, Schroeder RA, Wikman GK, Hancke JL (1997) Testicular toxicity assessment of Andrographis paniculata dried extract in rats. J Ethnopharmacol 58:219-224.
• Carrascosa JM, Molero JC, Fermin Y, Martinez C, Andres A, Satrustegui J (2001) Effects of chronic treatment with acarbose on glucose and lipid metabolism in obese diabetic Wistar rats. Diabetes Obes Metab 3:240-248.
• Gad MZ, El-Sawalhi MM, Ismail MF, El-Tanbouly ND (2006) Biochemical study of the anti-diabetic action of the Egyptian plants: Fenugreek and Balanites. Mol Cell Biochem 281:173-183.
• Georgetti SR, Casagrande R, Vicentini FTMC, Verri WA, Fonseca MJV (2006) Evaluation of the antioxidant activity of soybean extract by different in vitromethods and investigation of this activity after its incorporation in topical formulations. Eur J Pharm Biopharm 64:99-106.
• Handa SS, Sharma A (1990) Hepatoprotective activity of andrographolide from Andrographis paniculata against carbon tetrachloride. Indian J Med Res 92:276-283.
• Kim JS, Kwon CS, Son KH (2000) Inhibition of α-glucosidase and amylase by Luteolin, a flavonoid. Biosci Biotechnol Biochem 64:2458-2461.
• Kumar RA, Sridevi K, Vijaya Kumar N, Nanduri S, Rajagopal S (2004) Anticancer and immunostimulatory compounds from Andrographis paniculata. J Ethnopharmacol 92: 291-295.
• McCue P, Vattem D, Shetty K (2004) Inhibitory effect of clonal oregano extracts against porcine pancreatic amylase in vitro. Asia Pac J Clin Nutr 13:401-408.
• McCue P, Kwon YII, Shetty K (2005) Anti-diabetic and anti-hypertensive potential of sprouted and solid-state bioprocessed soybean. Asia Pac J Clin Nutr 14:145-152.
• Pullela SV, Tiwari AK, Vanka UMS, Vummenthula A, Tatipaka HB, Dasari KR, Khan IA, Janaswamy MR (2006) HPLC assisted chemobiological standardization of α-glucosidase-I enzyme inhibitory constituents from Piper longum Linn-An Indian medicinal plant. J Ethnopharmacol 108:445-449.
• Sheeja K, Guruvayoorappan C, Kuttan G (2007) Antiangiogenic activity of Andrographis paniculataextract and andrographolide. Int Immunopharmacol 7:211-221.
• Shen Y-C, Chen C-F, Chiou W-F (2002) Andrographolide prevents oxygen radical production by human neutrophils: possible mechanism(s) involved in its anti-inflammatory effect. Br J Pharmacol 135:399-406.
• Sima AAF, Chakrabarti S (2004) Long-term suppression of postprandial hyperglycaemia with acarbose retards the development of neuropathies in the BB/W-rat. Diabetologia 35:325-330.
• Singha PK, Roy S, Dey S (2007) Protective activity of andrographolide and arabinogalactan proteins from Andrographis paniculata Nees. against ethanol-induced toxicity in mice. J Ethnopharmacol 111:13-21.
• Tan BK, Zhang CY (1998) Pharmacological mechanisms of cardiovascular actions of diterpenoids of Andrographis paniculata. Invited Symposium. 5th Internet World Congress on Biomedical Sciences (INABIS), McMaster University, Canada, December 7-16.
• Ye F, Shen Z, Xie M (2002) Alpha-glucosidase inhibition from a Chinese medical herb (Ramulus mori) in normal and diabetic rats and mice. Phytomedicine 9:161-166.
• Yu BC, Hung CR, Chen WC, Cheng JT (2003) Antihyperglycaemic effect of andrographolide in streptozotocin-induced diabetic rats. Planta Medica 69:1075-1079.
• Zhang CY, Tan BK (1996) Hypotensive activity of aqueous extract of Andrographis paniculata in rats. Clin Exp Pharmacol Physiol 23:675-678.
• Zhang CY, Tan BK (1997) Mechanisms of cardiovascular activity of Andrographis paniculata in the anaesthetized rat. J Ethnopharmacol 56:97-101.
• Zhang CY, Kuroyangi M, Tan BK (1998) Cardiovascular activity of 14-deoxy-11,12-didehydroandrographolide in the anaesthetized rat and isolated rat atria. Pharmacol Res 38:413-417.
• Zhang XF, Tan BK (2000a) Anti-diabetic property of ethanolic extract of Andrographis paniculata in streptozotocin-diabetic rats. Acta Pharmacol Sin 21: 1157-1164.
• Zhang XF, Tan BK (2000b) Antihyperglycaemic and antioxidant properties of Andrographis paniculata in normal and diabetic rats. Clin Exp Physiol Pharmacol 27:358-363.