The influence of choleterol and biomass concentration on the uptake of cholesterol by Lactobacillus from MRS broth

• Autorzy: Ziarno M

Warianty tytułu

PL

Wplyw stezenia cholesterolu i biomasy na usuwanie cholesterolu przez bakterie rodzaju Lactobacillus z bulionu MRS

• Języki publikacji: EN

Abstrakty

EN

The aim of this study was the determination of some factors influence (i.e. the vitality of bacteria cells and the cholesterol concentration) on the ability of selected Lactobacillus sp. to cholesterol uptake during culture in MRS broth. Three Lactobacillus strains (Lb. delbrueckii subsp. bulgaricus, Lb. acidophilus, Lb. casei) isolated from commercial single species lyophilized dairy starter cultures and three Lactobacillus strains (Lb. plantarum, Lb. delbrueckii subsp. bulgaricus, Lb. acidophilus) originated from commercial pharmaceuticals were used in this study. The uptake of cholesterol from MRS broth during the growth of Lactobacillus sp., expressed as the difference between the final and the initial concentrations of cholesterol, ranged from 0.053 to 0.153 g/dm3, apart from the initial cholesterol content and the origin of Lactobacillus sp. The results confirmed that biomass concentration have a statistically significant effect on uptake of cholesterol. The ten-fold increase of the amount of intact cells biomass caused about 1.5-2-fold increase of the amount of cholesterol removed. The influence of the concentration of biomass of alive cells on the removal of cholesterol was bigger than in case of the heatsterilized cells.

PL

Celem pracy było określenie wpływu niektórych czynników (początkowego stężenia cholesterolu i żywotności komórek bakteryjnych) na zdolność wybranych pałeczek mlekowych do usuwania cholesterolu w bulionie MRS. W badaniach użyto trzy monokultury pałeczek mlekowych Lb. delbrueckii subsp. bulgaricus, Lb. acidophilus, Lb. casei wyizolowane z jednogatunkowych mleczarskich kultur starterowych oraz trzy monokultury pałeczek mlekowych Lb. plantarum, Lb. delbrueckii subsp. bulgaricus, Lb. acidophilus pochodzące z handlowych preparatów farmaceutycznych. Usuwanie cholesterolu bulionu MRS podczas rozwoju pałeczek, wyrażone jako różnica pomiędzy końcowym i początkowym stężeniem cholesterolu w podłożu, wynosiła od 0,053 do 0,153 g/dm3, niezależnie do początkowego stężenia cholesterolu i pochodzenia monokultur pałeczek. Wyniki potwierdziły, że stężenie biomasy komórkowej statystycznie istotnie wpływa na usunięcie cholesterolu. Dziesięciokrotne zwiększenie ilości biomasy martwych komórek skutkowało około 1,5-2-krotnym wzrostem ilości usuniętego cholesterolu. Wpływ koncentracji biomasy żywych komórek na usuwanie cholesterolu był większy niż w wypadku biomasy komórek zabitych termicznie.

Słowa kluczowe

EN

dairy starter culture; lactic acid bacteria; human nutrition; Lactobacillus; cholesterol uptake; cholesterol; biomass concentration

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2007
• Tom: 06
• Numer: 2
• Opis fizyczny: p.29-40,fig.,ref.

Twórcy

autor

Ziarno M

• Warsaw Agricultural University, Nowoursynowska 159C, 02-776 Warsaw, Poland

Bibliografia

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