Interactions of Yersinia enterocolitica biotype 4 and 1A strains with macrophages in vitro

• Autorzy: Kot B , Stepinska M. , Jakubczak A. , Trafny E.A.
• Języki publikacji: EN

Abstrakty

EN

The interaction of Y. enterocolitica strains belonging to 4 and 1A biotypes with RAW264.7 murine macrophage- like cell monolayers was studied. Y. enterocolitica strains from humans and pigs were characterized in terms of their internalization and survival within RAW264.7 cell monolayers and their ability to escape from the cells. Y. enterocolitica biotype 4 strains invaded macrophage cell monolayers to a significantly higher degree than biotype 1A strains. However, biotype 1A strains exhibited a greater level of survival in macrophages than biotype 4 strains. All Y. enterocolitica strains tested demonstrated the ability to escape from macrophages. The mechanisms that allow Y. enterocolitica biotype 1A to survive within macrophages may contribute to the virulence of these organisms

Słowa kluczowe

EN

human pathogen; Yersinia enterocolitica; interaction; biotype; macrophage; bacterial strain; growth condition; infection condition; survival; virulence

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 04
• Opis fizyczny: p.416-420,fig.,ref.

Twórcy

autor

Kot B

• University of Podlasie, 12 Boleslawa Prusa Str., 08-110 Siedlce, Poland

autor

Stepinska M.

autor

Jakubczak A.

autor

Trafny E.A.

Bibliografia

• 1.Bleves S., Cornelis G.: How to survive in the host: the Yersinia lesson. Microb. Infect. 2000, 2, 1451-1460.
• 2.Bottone E. J.: Yersinia enterocolitica: overview and epidemiologic correlates. Microb. Infect. 1999, 1, 323-333.
• 3.Brzostek K., Raczkowska A., Zasada A.: The osmotic regulator OmpR is involved in the response of Yersinia enterocolitica O:9 to environmental stress and survival within macrophages. FEMS Microbiol. Lett. 2003, 228, 265-271.
• 4.Cornelis G. R.: Yersinia type III secretion: send in the effectors. J. Cell. Biol. 2002, 158, 401-408. 5.Ellison D. W., Lawrenz M. B., Miller V.: Invasin and beyond: regulation of Yersinia virulence by RovA. Trends Microbiol. 2004, 12, 296-300.
• 6.Fredriksson-Ahomaa M., Korkeala H.: Low occurrence of pathogenic Yersinia enetrocolitica in clinical, food, and environmental samples: a methodological problem. Clin. Microbiol. Rev. 2003, 16, 220-229.
• 7.Fredriksson-Ahomaa M., Niskanen T., Bucher M., Stolle A., Korkeala H.: Different Yersinia enterocolitica 4:03 genotypes found in pig tonsils in Southern Germany and Finland. Syst. Appl. Microbiol. 2003, 1, 132-137.
• 8.Grant T., Bennett-Wood V., Robins-Browne R. M.: Identification of virulence- -associated characteristic in clinical isolates of Yersinia enterocolitica lacking classical virulence markers. Infect. Immun. 1998, 66, 1113-1120.
• 9.Grant T., Bennett-Wood V., Robins-Browne R. M.: Characterization of the interaction between Yersinia enterocolitica biotype 1A and phagocytes and epithelial cells in vitro. Infect. Immun. 1999, 67, 4367-4375.
• 10.Grosdent N., Maridonneau-Parini I., Sory M., Cornelis G. R.: Role of Yops and adhesions in resistance of Yersinia enterocolitica to phagocytosis. Infect. Immun. 2002, 70, 4165-4176. 11.Heesemann J., Sing A., Trülzsch K.: Yersinia's stratagem: targeting innate and adaptive immune defense. Curr. Opinion Microbiol. 2006, 9, 55-61.
• 12.Iriarte M., Cornelis G. R.: MyfF, an element of the network regulating the synthesis of fibrillae in Yersinia enterocolitica. J. Bacteriol. 1995, 177, 738- -744.
• 13.Kot B., Trafny E. A.: The application of PCR to the identification of selected virulence markers of Yersinia genus. Pol. J. Vet. Sci. 2004, 7, 27-31.
• 14.Kot B., WoŸniak-Kosek A., Kawiak J., Bukowski K.: Application of PCR for identification of pathogenic plasmid markers of Yersinia enterocolitica strains isolated from humans and pigs. Vet. Med. 2001, 57, 727-730.
• 15.McNally A., Cheasty T., Fearnley C., Dalziel R. W., Paiba G. A., Manning G., Newell D. G.: Comparison of the biotypes of Yersinia enterocolitica isolated from pigs, cattle and sheep at slaughter and from humans with yersiniosis in Great Britain during 1999-2000. Lett. Appl. Microbiol. 2004, 39, 103-108.
• 16.Pujol C., Bliska J. B.: Turning Yersinia pathogenesis outside in: subversion of macrophage function by intracellular yersiniae. Clin. Immun. 2005, 114, 216- -226.
• 17.Ramamurthy T., Yoshino K., Huang X., Nair G. B., Carniel E., Maruyama T., Fukushima H., Takeda T.: The novel heat-stable enterotoxin subtype gene (ystB) of Yersinia enterocolitica: nucleotide sequence and distribution of the yst genes. Microb. Pathog. 1997, 23, 189-200.
• 18.Rastawicki W., Gierczyñski R., Jagielski M., Kałużewski S., Jeljaszewicz J.: Susceptibility of Polish clinical strains of Yersinia enterocolitica serotype O3 to antibiotics. Intern. J. Antimicrob. Agents 2000, 13, 297-300.
• 19.Revell P. A., Miller V. L.: Yersinia virulence: more than a plasmid. FEMS Microbiol. Lett. 2001, 205, 159-164.
• 20.SAS Institute. SAS user’s guide. Statistics. 6th ed. SAS Institute, Cary, NC 1988.
• 21.Singh I., Virdi J. S.: Interaction of Yersinia enterocolitica biotype 1A strains of diverse origin with cultured cells in vitro. Jpn. J. Infect. Dis. 2005, 58, 31-33.
• 22.Tennant S. M., Grant T. H., Robins-Browne R. M.: Pathogenicity of Yersinia enterocolitica biotype 1A. FEMS Immun. Med. Microbiol. 2003, 38, 127-137.
• 23.Thoerner P., Bin Kingombe C. I., Bögli-Stuber K., Bissig-Choisat B., Wassenaar T. M., Frey J., Jemmi T.: PCR detection of virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virulence gene distribution. Appl. Environ. Microbiol. 2003, 69, 1810-1816.
• 24.Wauters G., Kandolo K., Janssens M.: Revised biogrouping scheme of Yersinia enterocolitica. Contrib. Microbiol. Immunol. 1987, 9, 14-21.
• 25.Wożniak-Kosek A., Kot B., Jakubczak A., Grzybowski J.: Application of PCR for the identification of pathogenic Yersinia enterocolitica strains isolated from humans and pigs. Vet. Med. 2000, 57,183-185.