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2006 | 62 | 09 |

Tytuł artykułu

Kontrola mieszanki paszowej na obecnosc komponentow pochodzenia zwierzecego

Warianty tytułu

EN
Methods of detecting animal components in feedstuff

Języki publikacji

PL

Abstrakty

EN
Epizootiological studies have shown that meat-and-bone meal of ovine or bovine origin added to feed concentrates are infectious agents in bovine spongiform encephalopathy (BSE). In view of the ban on the use of meat-and-bone meal in animal feed in European Union countries, the aim of the study was to elaborate methods for identifying chicken and sheep DNA in compound feeds. A duplex PCR reaction (simultaneous amplification of chicken and sheep DNA) was used to identify mtDNA specific to chickens and sheep. The reaction products were subjected to electrophoresis in 4% agarose gel in the presence of X 174DNA/HaeIII marker. The results obtained enabled the identification of DNA specific to both chickens and sheep.

Wydawca

-

Rocznik

Tom

62

Numer

09

Opis fizyczny

s.1033-1034,rys.,bibliogr.

Twórcy

  • Instytut Zootechniki, ul.Krakowska 1, 32-083 Balice
autor
autor

Bibliografia

  • 1. Boom R., Sol C. J. A., Salimans M. M. M., Jansen C. L., van Dillen P. M. E., Wertheim J., van der Noordaa J.: Rapid and simple method or purification of nucleic acids. J. Clin. Microbiol. 1990, 28, 495-503.
  • 2. Boom R., Cees S., Beld M., Weel J., Goudsmit J., van Dillen P. W.: Improved silica-guanidiniumthiocyanate DNA isolation procedure based on selective binding of bovine alpha-casein to silica particles. J. Clin. Microbiol. 1999, 37, 615-619.
  • 3. Hahn H.: Animal meal: production and determination in feedstuffs and the origin of bovine spongiform encephalopathy. Naturwissenschaften. 1999, 86, 62-70.
  • 4. Institute for Reference Materials and Measurement. Animal by-products in animal feed. November 2003.
  • 5. Lahiff S., Glennon M., O'Brien L., Lyng J., Smith T., Maher M., Shilton N.: Species-specific PCR for the identification of ovine, porcine and chicken species in meat and bone meal (MBM). Mol. Cell. Probes 2001, 15, 27-35.
  • 6. Lahiff S., Glennon M., Lyng J., Smith T., Shilton N., Maher M.: Real-time polymerase chain reaction detection of bovine DNA in meat and bone meal samples. J. Food Prot. 2002, 65, 1158-1165.
  • 7. Natonek M., Słota E., Żyga A., Rejduch B.: The utilization of methods based on protein and DNA analysis for identification of animal-origin components in feeds. J. Anim. Feed Sci. 2004, 13, 73-76.
  • 8. Polak M., Larska M., Żmudziński J.: Nowe szybkie testy do diagnostyki post mortem BSE. Med. Wet. 2003, 10, 876-878.
  • 9. Polak M., Rożek W., Żmudziński J.: Monitoring BSE w Polsce. Med. Wet. 2002, 5, 344-347.
  • 10. Tartaglia M., Saulle E., Pestalozza S., Morelli R., Antonucci G., Bataglia P. A.: Detection of Bovine mitochondrial DNA in ruminant feeds: a molecular approach to test for the presence of bovine-derived materials. J. Food Prot. 1997, 61, 513-518.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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