3'Noncoding sequences of the CTA 1 gene enhance expression of the recombinant serine protease inhibitor, CPTI II, in Saccharomyces cerevisiae

• Autorzy: Stankiewicz M. , Rempola B. , Fikus M.
• Języki publikacji: EN

Abstrakty

EN

Expression of the gene coding for the recombinant trypsin inhibitor, CPTI II, was enhanced tenfold when yeast transcription terminating sequences were added to the expression cassette of the pJK6 yeast vector. The yield was further increased about 20% in the BJ5464 yeast strain, defective in vacuolar proteases.

Słowa kluczowe

EN

squash seed; yeast vector; serine protease; vacuolar protease; gene coding; recombinant trypsin inhibitor; inhibitor; Cucurbita pepo; yeast cell; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1996
• Tom: 43
• Numer: 3
• Opis fizyczny: p.525-529,fig.

Twórcy

autor

Stankiewicz M.

• Polish Academy of Sciences, A.Pawinskiego 5a, 02-106 Warsaw, Poland

autor

Rempola B.

autor

Fikus M.

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