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2006 | 13 | 1 |

Tytuł artykułu

PCR detection of granulocytic Anaplasma and Babesia in Ixodes ricinus ticks and birds in West-Central Poland

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of the study was to establish the role of forest birds as reservoirs of Anaplasma phagocytophilum and Babesia spp. in Wielkopolski National Park. A total of 108 birds from 9 species were collected between May–September 2002. Blood samples were taken from 84 specimens and 442 individuals of the common tick, Ixodes ricinus, were collected from the birds. The 73 additional ticks were collected from vegetation. PCR amplification of a fragment of the epank 1 gene and 18S rRNA gene was used for detection of A. phagocytophilum and Babesia spp. DNA, respectively. Pathogen DNA was not detected in any of the blood samples or ticks collected from birds. On the other hand, 3 ticks collected from vegetation (4.1% of all examined specimens) were positive for A. phagocytophilum DNA. In spite of the high level of infestation of birds by I. ricinus, it is clear that they do not constitute a competent reservoir of A. phagocytophilum and Babesia in WNP. Additionally, I. ricinus is not a significant vector in this area.

Wydawca

-

Rocznik

Tom

13

Numer

1

Opis fizyczny

p.21-23,fig.,ref.

Twórcy

  • Szczecin University, Al.Piastow 40B, 71-065 Szczecin, Poland
autor
autor
autor

Bibliografia

  • 1. Alberdi MP, Walker AR, Urquhart KA: Field evidence that roe deer (Capreolus capreolus) are a natural host for Ehrlichia phagocytophila. Epidemiol Infect 2000, 124(2), 315-323.
  • 2. Alekseev AN, Dubinina HV, Van De Pol I, Schouls LM: Identification of Ehrlichia spp. and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia. J Clin Microbiol 2001, 39, 2237-2242.
  • 3. Bjöersdorff A, Bergstrom S, Massung RF, Haeming PD, Olsen B: Ehrlichia- infected ticks on migrating birds. Emerg Infect Dis 2001, 7, 877-879.
  • 4. Bown KJ, Begon M, Bennett M, Woldehiwet Z, Ogden NH: Seasonal dynamics of Anaplasma phagocytophila in a rodent-tick (Ixodes trianguliceps) system, United Kingdom. Emerg Infect Dis 2003, 9(1), 63-70.
  • 5. Cinco M, Padovan D, Murgia R, Maroli M, Frusteri L, Heldtander M, Johansson KE, Olsson-Engvall E: Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing. J Clin Microbiol 1997, 35, 3365-3366.
  • 6. Derdakova M, Halanova M, Stanko M¤, Štefančikova A, Čislakova L, Pet'kov B: Molecular evidence for Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Eastern Slovakia. Ann Agric Environ Med 2003, 10, 269-271.
  • 7. Dumler JS, Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR: Reorganisation of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales. Int J Syst Evol Microbiol 2001, 6, 2145-2165.
  • 8. Fingerle V, Munderloh UG, Liegl G, Wilske B: Coexistence of ehrlichiae of the phagocytophila group with Borrelia burgdorferi in Ixodes ricinus from Southern Germany. Med Microbiol Immunol 1999, 188, 145-149.
  • 9. Grzeszczuk A, Stanczak J, Kubica-Biernat B: Serological and molecular evidence of human granulocytic ehrlichiosis focus in the Białowieża Primeval Forest (Puszcza Białowieska), northeastern Poland. Eur J Clin Microbiol Infect Dis 2002, 21, 6-11.
  • 10. Guy E, Tasker S, Joynson DHM: Detection of the agent of human granulocytic ehrlichiosis (HGE) in UK tick using polymerase chain reaction. Epidemiol Infect 1998, 121, 681-683.
  • 11. Homer MJ, Aguilar-Delfin I, Telford III SR, Krause PJ, Persing DH: Babesiosis. Clin Microbiol Rev 2000, 13, 451-469.
  • 12. Michalik J, Skotarczak B, Skoracki M, Wodecka B, Sikora B, Hofman T, Rymaszewska A, Sawczuk M: Borrelia burgdorferi sensu stricto in yellow-necked mice and feeding Ixodes ricinus ticks in a forest habitat of west central Poland. J Med Entomol 2005, 42(5), 850-856.
  • 13. Ogden NH, Bown K, Horrocks BK, Woldehiwet Z, Bennett M: Granulocytic Ehrlichia infection in Ixodid ticks and mammals in woodlands and uplands of the UK. Med Vet Entomol 1998, 12, 423-429.
  • 14. Peirce MA: A taxonomic review of avian piroplasms of the genus Babesia Starcovici, 1893 (Apicomplexa: Piroplasmorida: Babesiidae). J Natural History 2000, 34, 317-332.
  • 15. Skotarczak B, Cichocka A: The occurrence DNA of Babesia microti in ticks Ixodes ricinus in the forest areas of Szczecin. Folia Biol (Kraków) 2001 a, 49, 247-250.
  • 16. Skotarczak B, Cichocka A: Isolation and amplification by polymerase chain reaction DNA of Babesia microti and Babesia divergens in ticks in Poland. Ann Agric Environ Med 2001, 8, 187-189.
  • 17. Skotarczak B, Rymaszewska A, Sawczuk M, Adamska M, Maciejewska A, Jerszynska-Mazur J: Search for granulocytic Anaplasma and Babesia in Ixodes ricinus ticks and yellow-necked mice (Apodemus flavicolis) in West-Central Poland. Vet Med Czech, in press.
  • 18. Skotarczak B. Rymaszewska A: Wstępne badania czynnika etiologicznego ludzkiej ehrlichiozy (HGE) agent DNA in Ixodes ricinus ticks. Folia Med Cracoviensia 2003, 1-2, 205-211. 19. Skotarczak B, Rymaszewska A, Adamska M: Polymerase chain reaction in detection of human granulocytic ehrlichiosis (HGE) agent DNA in Ixodes ricinus ticks. Folia Med Cracoviensia 2003, 1-2, 205-211.
  • 20. Stańczak J, Racewicz M, Kruminis-Łozowska W, Kubica-Biernat B: Coinfection of Ixodes ricinus (Acari: Ixodidae) in northern Poland with the agents of Lyme borreliosis (LB) and human granulocytic ehrlichiosis (HGE). Int J Med Microbiol 2002, 291, 198-201.
  • 21. Walls JJ, Caturegli P, Bakken JS, Asanovich KM, Dumler JS: Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epank 1 genes of Ehrlichia phagocytophila – group Ehrlichiae. J Clin Microbiol 2000, 38, 354-356.

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Bibliografia

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