Cross-species amplification of microsatellite markers in the invasive spiny-cheek crayfish [Orconectes limosus]: assessment and application

• Autorzy: Hulak M , Kaspar V , Kozak P , Buric M , Filipova L , Petrusek A
• Języki publikacji: EN

Abstrakty

EN

The North American spiny-cheek crayfish, Orconectes limosus (Cambaridae), endangered in its native range, is a widespread invasive species in European waters and conservationally important carrier of crayfish plague. However, its population structure is poorly known, and no informative genetic markers for the species are available. We tested cross-species transfer of microsatellite loci to spiny-cheek crayfish from 5 other crayfish species. Variability of 10 successfully amplifying loci derived from 4 species was then tested in 60 individuals of O. limosus originating from 3 natural populations: the river Danube at Bogyiszló in Hungary, a pond in Starý Klíčov, and the brook Černovický, both in the Czech Republic. The allele number within the populations ranged from 4 to 10 alleles per locus, while heterozygosity levels varied from 0.650 to 0.900 for Ho and from 0.660 to 0.890 for He. No linkage disequilibrium and no null alleles were detected. The selected markers are useful for assessing population structure, intraspecific variation, and paternity studies in spiny-cheek crayfish.

Słowa kluczowe

PL

spiny-cheek crayfish; cross-species testing; repetitive element; crayfish; invasive species; Orconectes limosus; population genetics; microsatellite marker

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2010
• Tom: 51
• Numer: 1
• Opis fizyczny: p.73-78,ref.

Twórcy

autor

Hulak M

• Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Zatisi 728/II, Vodnany, CZ-38925, Czech Republic

autor

Kaspar V

• Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Zatisi 728/II, Vodnany, CZ-38925, Czech Republic

autor

Kozak P

• Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Zatisi 728/II, Vodnany, CZ-38925, Czech Republic

autor

Buric M

• Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Zatisi 728/II, Vodnany, CZ-38925, Czech Republic

autor

Filipova L

• Faculty of Science, Department of Ecology, Charles University in Prague 2, Czech Republic

autor

Petrusek A

• Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, Zatisi 728/II, Vodnany, CZ-38925, Czech Republic
• Faculty of Science, Department of Ecology, Charles University in Prague 2, Czech Republic

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