Cloning and characterization of the gene encoding ribosomal PO phosphoprotein from Neurospora crassa

• Autorzy: Krokowski D. , Tchorzewski M. , Grankowski N.
• Języki publikacji: EN

Abstrakty

EN

A gene for ribosomal protein P0 that belongs to the family of ribosomal P proteins was isolated from a Neurospora crassa cDNA library, using polyclonal antibodies against recombinant P0 protein from Saccharomyces cerevisiae. This is the first gene for ribosomal P0 protein to be cloned from filamentous fungi. The derived P0 protein sequence has a strong homology to other eukaryotic P0 proteins; yet, there is a nota­ble alteration in the conservative C-terminal region, placing this protein among the unique sequences from protozoan parasites.

Słowa kluczowe

EN

phosphorylation; cloning; gene encoding; Neurospora crassa; phosphoprotein; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2002
• Tom: 49
• Numer: 1
• Opis fizyczny: p.11-18,fig.

Twórcy

autor

Krokowski D.

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Tchorzewski M.

autor

Grankowski N.

Bibliografia

• 1.Rodriguez-Gabriel,M.A., Remacha, M. & Ballesta, J.P. (2000) The RNA interacting domain but not the protein interacting domain is highly conserved in ribosomal protein P0. J. Biol. Chem. 275, 2130-2136.
• 2.Shimmin, L.C., Ramirez, C., Matheson, A.T. & Dennis, P.P. (1989) Sequence alignment and evolutionary comparison of the L10 equivalent and L12 equivalent ribosomal proteins from archaebacteria, eubacteria, and eucaryotes. J. Mol. Evol. 29, 448-462.
• 3.Boguszewska, A., Szyszka, R. & Grankowski, N. (1997) The phosphorylation sites of ribosomal P proteins from S. cerevisiae cells by endogenous CK-2, PK60S and RAP protein kinases. Acta Biochim. Polon. 44, 191-200.
• 4.Santos, C. & Ballesta, J.P. (1994) Ribosomal protein P0, contrary to phosphoproteins P1 and P2, is required for ribosome activity and Saccharomyces cerevisiae viability. J. Biol. Chem. 269, 15689-15696.
• 5.Remacha,M., Santos, C. & Ballesta, J.P. (1995) Disruption of single-copy genes encoding acidic ribosomal proteins in Saccharomyces cerevisiae. Mol. Cell. Biol. 10, 2182-2190.
• 6.Uchiumi,T., Kikuchi, M., Terao, K., Iwasaki, K.N. & Ogata, K. (1986) Cross-linking of elongation factor 2 to rat-liver ribosomal proteins by 2-iminothiolane. Eur. J. Biochem. 156, 37-48.
• 7.Grabowski,D.T., Deutsch, W.A., Derda, D. & Kelley, M.R. (1991) Drosophila AP3, a presumptive DNA repair protein, is homologous to human ribosomal associated protein P0. Nucleic Acids Res. 19, 4297.
• 8.Frolov,M.V. & Birchler, J.A. (1998) Mutation in P0, a dual function ribosomal protein/ apurinic/apyrimidinic endonuclease, modifies gene expression and position effect variegation in Drosophila. Genetics 150, 1487-1495.
• 9.Hulsey,M., Goldstein, R., Scully, L., Surbeck, W. & Reichlin, M. (1995) Anti-ribosomal P antibodies in systemic lupus erythematosus: A case-control study correlating hepatic and renal disease. Clin. Immunol. Immunopathol. 74, 252-256.
• 10.Schijman, A.G., Levitus, G. & Levin, M.J. (1992) Characterization of the C-terminal region of a Trypanosoma cruzi 38- kDa ribosomal P0 protein that does not react with lupus anti-P autoantibodies. Immunol. Lett. 33, 15-20.
• 11.Kurup,V. & Banerjee, B. (2000) Fungal allergens and peptide epitopes. Peptides 21, 589-599.
• 12.Kusuda, M., Yajima, H. & Inoue, H. (2000) Characterization and expression of a Neurospora crassaribosomal protein gene, crp-7. Curr. Genet. 37, 119-124.
• 13.Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning. A Laboratory Manual.Cold Spring Harbor Laboratory. Cold Spring Harbor NY.
• 14.Garber, R.C. & Yoder, C.O. (1983) Isolation of DNA from filamentous fungi and separation into nuclear, mitochondrial, ribosomal, and plasmid components. Anal. Biochem. 135, 416-422.
• 15.Sodeoka, M., Larson, C.J., Chen, L., LeClair, K.P. & Verdine, G.L. (1993) Multifunctional plasmid for protein expression by ECPCR. Overproduction of the p50 subunit of NF-kappa-B. Bioorg. Med. Chem. Lett. 3, 1089-1095.
• 16.Tchorzewski,M., Boguszewska, A., Abramczyk, D. & Grankowski, N. (1999) Overexpression in Escherichia coli, purification, and characterization of recombinant 60S ribosomal acidic proteins from Saccharomyces cerevisiae. Protein Expr. Purif. 15, 40-47.
• 17.Laemmli,U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature. 227, 680-685.
• 18.Altschul,S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
• 19.Thompson,J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.
• 20.Rambosek,J. & Leach, J. (1987) Recombinant DNA in filamentous fungi: Progress and prospects. Crit. Rev. Biotechnol. 6, 357-393.
• 21.Planta,R.J. & Mager, W.H. (1998) The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast. 14, 471-477.
• 22.Santos, C. & Ballesta, J.P. (1995) The highly conserved protein P0 carboxyl end is essential for ribosome activity only in the absence of proteins P1 and P2. J. Biol. Chem. 270, 20608-20614.