Molecular characteristics of sucrose synthase isolated from bird cherry leaves

• Autorzy: Sytykiewicz H , Czerniewicz P. , Leszczynski B.

Warianty tytułu

PL

Charakterystyka molekularna syntazy sacharozy izolowanej z lisci czeremchy zwyczajnej

• Języki publikacji: EN

Abstrakty

EN

The molecular characteristics of partly purified sucrose synthase (NDP-glucose: D-fructose 2-α-D-glucosyltransferase, EC 2.4.1.13), extracted from the bird cherry (Prunus padus L.) leaves was elucidated. The sucrose synthase was successfully purified by using a four-step protocol including ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-150 and ion-exchange chromatography with the use of DEAE-cellulose. The analysed enzyme occurred in two isoforms (SuSyI and SuSyII). The relative molecular weight of native isoenzymes was estimated to be 200 and 180 kDa, respectively. Isoform SuSyI contained two different subunits of 57.5 and 52.8 kDa, whereas the structure of SuSyII was consisted of identical 63 kDa subunits. Experimental data indicated that the structure of both SuSy isoforms was composed of three subunits

PL

W przeprowadzonych badaniach dokonano charakterystyki molekularnej częściowo oczyszczonej syntazy sacharozy (NDP-glukoza: D-fruktoza 2-α-D-glukozylotransferaza, EC 2.4.1.13), ekstrahowanej z liści czeremchy zwyczajnej (Prunus padus L.). Syntaza sacharozy została oczyszczona w wysokim stopniu przy wykorzystaniu czteroetapowej procedury obejmującej precypitację siarczanem amonu, dializę, filtrację żelową na złożu Sephadex G-150 i chromatografię jonowymienną na złożu DEAE-celuloza. Analizowany enzym występował w postaci dwóch izoenzymów (SuSyI i SuSyII). Względny ciężar cząsteczkowy natywnych form analizowanego enzymu wynosił odpowiednio 200 i 180 kDa. Izoforma SuSyI zawierała w swym składzie dwie różne podjednostki (57,5 i 52,8 kDa), podczas gdy struktura SuSyII składała się z monomerów o ciężarze cząsteczkowym 63 kDa. Przeprowadzone badania wskazują, że obydwie izoformy SuSy zawierały po trzy podjednostki.

Słowa kluczowe

EN

sucrose synthase; Prunus padus; subunit composition; bird cherry; leaf; molecular mass

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2008
• Tom: 54
• Numer: 3
• Opis fizyczny: p.41-49,fig.,ref.

Twórcy

autor

Sytykiewicz H

• University of Podlasie, Prusa 12, 08-110 Siedlce, Poland

autor

Czerniewicz P.

autor

Leszczynski B.

Bibliografia

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