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2002 | 48 | 2 |

Tytuł artykułu

Influence of Anisakis simplex stage III larvae upon the activity of proteases under in vitro conditions

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The larvae of Anisakis simplex had the largest influence upon decreasing the activity of porcine pepsin. The activity of that enzyme in tests, where the larvae were present during the entire period of incubation, was lower than in the controls. No similar trends were observed in case of the solutions with bovine and porcine trypsin. The activity of those enzymes in the solutions containing the larvae was higher than in the controls. Only the activity of porcine trypsin after 10 h of incubation was slightly lower in the experimental sample than in the control, however, during the later hours the dynamics of the activity decrease of that enzyme in the controls was higher than in the experimental samples. The recorded activity of papain in the samples containing the larvae was higher than that in the controls during the entire time of the experiment.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

48

Numer

2

Opis fizyczny

p.217-223,fig.,ref.

Twórcy

  • University of Warmia and Mazury, Zolnierska Str.14, 10-561 Olsztyn, Poland
autor

Bibliografia

  • Abu-Erreish G., Peanasky R.J. 1974a. Pepsin inhibitors from Ascaris lumbricoides. Isolation, purification, and some properties. Journal of Biological Chemistry 249: 1558-1565.
  • Abu-Erreish G., Peanasky R.J. 1974b. Pepsin inbibitors from Ascaris lumbricoides. Pepsiniohibitor complex: stoichiometry of formation, dissociation, and stability of the complex. Journal of Biological Chemistry 249: 1566-1571.
  • Ansari A.A., Khan M.A., Ghatak S. 1976. Ascaridia galli: trypsin and chymotrypsin inbibitors. Experimental Parasirology 39: 74-83.
  • Dziekońska-Rynko J., Rokicki J., Jabłonowski Z. 1997a. The impact of animal and plant proteases on nematoda 3rd stage Anisakis simplex larvae. Oceanological Studies 1: 103-107.
  • Dziekońska-Rynko J., Rokicki J., Jabłonowski Z. 1997b. The influence of infection with III larval stage of Anisakis simplex on the activity of proteases in alimentary tract of guinea pigs. Wiadomości Parazytologiczne 43: 425-430.
  • Green N.M. 1957. Chymotrypsin inhibitor from Ascaris. Biochemical Journal 66: 416-418.
  • Hawley J.H., Peanasky R. 1992. Ascaris suum: are trypsin inhibitors involved in species specificity of Ascarid Nematodes? Experimental Parasitology 75: 112-118.
  • Hotez P.J., Cappello M., Hawdon J., Beckers C., Sakanari J. 1994. Hyaluronidases of the gastrointestinal invasive nematodes Ancylostoma caninum and Anisakis simplex: Possible functions in the pathogenesis of human zoonoses. Journal of Infections Diseases 170: 918-926.
  • Juhasz S. 1979. Proteolytic enzymes and enzyme inhibitors in Ascaris suum. II. Proteinase inhibitors produced in vitro in enzyme-free medium. Acta Veterinaria Academiae Scientiarium Hungaricae 27: 89-91.
  • Juhasz S., Matskasi 1. 1979. Proteolytic enzymes and enzyme inhibitors in Ascaris suum. III. In vitro study of proteinase inactivation by the parasite. Acta Vererinaria Academiae Scienriarium Hungarica 27: 93-97.
  • Kadłubowski R., Ochęcka A., Wójcik A., Błaszkowska J. 1991/ 1992. Proteolysis inhibitors from Ascaris lumbricoides: fact or fiction? Wiadomosci Parazytologiczne 37: 423-432.
  • Kłyszejko-Stefanowicz L. 1982. Practice of Biochemistry. Warszawa-Poznań, PWN. 182-183.
  • Matthews B. 1982. Behaviour and enzyme release by Anisakis sp. larvae (Nematoda: Ascaridida). Journal of Helminthology 56: 177- 183.
  • Matthews B. 1984. The source, release and specificity of proteolytic enzyme activity produced by Anisakis simplex larvae (Nematoda: Ascaridida) in vitro. Journal of Helminthology 58: 175-185.
  • Morris S.R., Sakanari J. A. 1994. Characterization of the serine protease and serioe protease inhibitor from the tissue-penetrating Nematode Anisakis simplex. Journal of Biological Chemistry 269: 27650-27656.
  • Opperman C.H., Chang S. 1992. Nematode acetylcholinesterases: molecular forms and their potential role in Nematode behavior. Parasitology Today 8: 406-411.
  • Pappas P.W., Read C.P. 1972a. Trypsin inactivation by intact Hymenolepis diminuta. Journal of Parasitology 58: 864-871.
  • Pappas P.W., Read C.P. 1972b. Inactivation of α and β chymotrypsin by intact Hymenolepis diminuta. Biological Bulletin 143: 605-616.
  • Peanasky R.J., Laskowski M. 1960. Protease inhibitors from Ascaris lumbricoides. Biochimica et Biophysica Acta 37: 167-169.
  • Sakanari J.A., McKerrow J.H. 1990. Identification of the secreted neutral proteases from Anisakis simplex. Journal of Parasitology 76: 625-630.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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