PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 58 | 2 |

Tytuł artykułu

Changes in microbial community structure during adaptation towards polyhydroxyalkanoates production

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Polyhydroxyalkanoates (PHAs) are interesting as material for bioplastic production because they are recognized as biodegradable and could be produced from renewable resources. The industrial production of PHAs has already been used in practice by pure cultures. In recent years, many studies have been addressed of PHA production by mixed cultures. Nevertheless, while fermentation strategy to improve the PHA content of biomass, yield and productivity in pure cultures are well defined, knowledge about the operational condition for PHA synthesis by mixed culture is still very limited. The ecology of the microbial community of activated sludge remains largely unknown, primarily because of the difficulty of making detailed observation. Recently, developed molecular techniques allow determination of community composition from DNA extracted directly from biomass samples. This study examined the changes of bacterial communities in activated sludge through application of the molecular technique, ribosomal intergenic spacer analysis (RISA). Microbial communities from anaerobic-aerobic and ammonia limited fermentations were ascertained. The applied operational conditions were shown to select for a restricted microbial population, which were different in term of structure with respect to the initial microbial consortia in the activated sludge used as inoculum.

Wydawca

-

Rocznik

Tom

58

Numer

2

Opis fizyczny

p.131-139,fig.,ref.

Twórcy

autor
  • University of Warmia and Mazury in Olsztyn, Sloneczna 45G, 10-709 Olsztyn, Poland
autor
autor
autor

Bibliografia

  • Blackwood C.B., T. Marsh, S-H. Kim and E.A. Paul. 2003. Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communities. Appl. Environ. Microbiol. 64: 926-932.
  • Bormann E.J., M. Leiβer and B. Beer.1998. Growth- associated production of poly (hydroxybutric acid) by Azotobacter beijerlnckii from organic nitrogen substrates. Appl. Microbiol. Biotechnol. 49: 84-88.
  • Borneman J. and E.W. Triplett. 1997. Molecular microbial diversity in soils from eastern Amazonia evidence for unusual microorganisms and microbial population shifts associated with deforestation. Appl. Environ. Microbiol. 63: 2647-2653.
  • Dias J.M.L., P.C. Lemos, L.S. Serafïm, C. Oliveira, M. Eiroa, M.G.E. Albuquerque, A.M. Ramos, R. Oliveir and M.A.M. Reis. 2006. Recent advances in polyhydroxyalkanoate production by mixed aerobic cultures: From the substrate to the final product. Macromol. Biosci. 6: 885-906.
  • Dionisi D., M. Beccari, S. Di Gregorio, M. Majone, M.P. Papini and G. Vallini. 2005. Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate. J. Chem. Teehnol. Biotechnol. 80: 1306-1318.
  • Dionisi D., M. Majone, G. Vallini, S. Di Gregorio and M. Beccari. 2006. Effect of the applied organic load rate on biodegradable polymer production by mixed microbial cultures in a sequencing batch reactor. Biotechnol. Bioeng. 93: 76-88.
  • Dolzani L., E. Tonin, C. Lagatolla, L. Prandin and C. Monti-Bragadin. 1996. Identification of Acinetobacter isolates in the A. calcoaceticus-A. baumannii complex by restriction analysis of the 16S-23S rRNA intergenic-spacer sequences. J. Clin. Microbiol. 33: 1108-1113.
  • Gürtler V. and V.A. Stanisich. 1996. New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology 142: 3-16.
  • Hewson I. and J.A. Fuhrman. 2004. Richness and diversity of bacterioplankton species along an estuarine gradient in Moreton Bay, Australia. Appl. Environ. Microbiol. 70: 3425-3433.
  • Kadouri D., E. Jurkevitch, Y. Okon and S. Castro-Sowinski. 2005. Ecological and agricultural significance of bacterial polyhydroxyalkanoates. Crit. Rev. Microbiol. 31: 55-67.
  • Kleerebezem R. and M.C.M. van Loosdrecht M.C.M. 2007. Mixed culture biotechnology for bioenergy production. Curr. Opin. Biotechnol. 18: 207-212.
  • Madison L.L. and G.W. Huisman. 1999. Metabolic engineering of poly (3-hydroalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63: 21-53.
  • Magurran A.E. 1988. Ecological Diversity and its Measurement. Princeton University Press.
  • Majone M., K. Dircks and J.J. Beun. 1999. Aerobic storage under dynamic conditions in activated sludge processes. The state of the art. Wat. Sci. Tech. 39: 61-73.
  • Mino T. M.C.M. Loosdrecht and J.J. Heijnen. 1998. Microbiology and biochemistry of the enhanced biological phosphate removal process. Wat. Res. 32: 3193-3207.
  • Muyzer G., E.C. de Waal and A.G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes encoding for 16S rRNA. Appl. Environ. Microbiol. 59: 695-700.
  • Nei M. and W.H. Li. 1997. Mathematical model for studying genetic variation in terms of restiction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273.
  • Øvreås L. 2000. Population and community level approaches for analysing microbial diversity in natural environments. Ecol. Lett. 3: 236-251.
  • Satoh H., T. Mino and T. Matsuo. 1992. Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes. Wat. Sci. Teehnol. 26: 933-942.
  • Satoh H., T. Mino and T. Matsuo. 1994. Deterioration of enhanced biological phosphorus removal by the domination of microorganisms without polyphosphate accumulation. Wat. Sci. Technol. 6: 203-211.
  • Shannon C.E. and W. Weaver. 1963. The Mathematical Theory of Communication. Urbana, University of Illinois Press.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-article-d2e386f7-e3b3-40d9-abab-4ff6fc154392
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.