Morphological and histological aspects of 2,4-D effects on rape explants [Brassica napus L. cv. Kana] cultured in vitro

• Autorzy: Slesak H , Popielarska M. , Goralski G.
• Języki publikacji: EN

Abstrakty

EN

The effect of different durations of exposure to 2,4-D on hypocotyls and cotyledons cultured in vitro was studied in Brassica napus L. cv. Kana. Organogenesis or callogenesis depended on the duration of explant exposure to MS medium supplemented with 2 mg/1 2,4-D (2,4-dichlorophenoxyacetic acid). Short treatment (1 and 3 days) with 2,4-D resulted in rhizogenesis on hypocotyls (100% and 98% of explants, respectively) and cotyledons (80% and 54% of explants, respectively). Adventitious shoots formed sporadically, with the highest frequency (14% of explants) on hypocotyls cultured 3 days on MS supplemented with 2,4-D and then transferred to hormone-free medium. Histological analysis clearly indicated that the basal part of hypocotyls is involved in root formation and callus production, and the apical part for shoots. Meristematic sites originated from groups of cells in the cortex layer (including cells of the endoderm), but the procambium, phloem and pericycle also showed meristematic activity. The present study indicated that the response of explants cultured on media containing 2,4-D at constant concentration depends on the duration of explant exposure to growth regulator.

Słowa kluczowe

EN

histology; Kana cultivar; plant cytology; callus; Brassica napus; regeneration; rape explant; morphology; 2,4-dichlorophenoxyacetic acid

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2005
• Tom: 47
• Numer: 1
• Opis fizyczny: p.219-226,fig.,ref.

Twórcy

autor

Slesak H

• Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland

autor

Popielarska M.

autor

Goralski G.

Bibliografia

• Angelini RR, and Allavena A. 1989. Plant regeneration from immature cotyledon explant cultures of bean (P. coccineus). Plant Cell, Tissue and Organ Culture 19: 167-174.
• Anzidei M, Bennici A, Schiff S, Tani C, and Mori B. 2000. Organogenesis and somatic embryogenesis in Foeniculum vulgare: histological observations of developing embryogenic callus. Plant Cell, Tissue and Organ Culture 61: 69-79.
• Bach A, and Pawłowska B. 2003. Somatic embryogenesis in Gentiana pneumonanthe L. Acta Biologica Cracoviensia Series Botanica 45: 79-86.
• Bagniewska-Zadworna A, Cegielska-Taras T, and Zenkteler M. 2001. Organogenesis initiation and plant regeneration from hypocotyls and cotyledons of androgenic embryos of Brassica napus L. Acta Biologica Cracoviensia Series Botanica 43: 51-57.
• Bogunia H, and Przywara L. 2000. Effect of carbohydrates on callus induction and regeneration ability in Brassica napus L. Acta Biologica Cracoviensia Series Botanica 42: 79-86.
• Bronsema FBF, van Oostveen, and van Lammeren AAM. 1997. Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632. Plant Cell, Tissue and Organ Culture 50: 57-65.
• Choi PS, Min SR, Ahn MY, Soh WY, and Liu JR. 1998. Somatic embryogenesis and plant regeneration in immature zygotic embryo, ovule, and anther filament cultures of chínese cabbage. Scientia Horticulturae 72: 151-155.
• Dietert MF, Barron SA, and Yoder OC. 1982. Effects of genotype on in vitro culture in the genus Brassica. Plant Science Letters 26: 233-240.
• Emons AMC, Samallo-Droppers, and van Der Torn C. 1993. The influence of sucrose, mannitol, L-proline, abscisic acid and gibberellic acid on the maturation of somatic embryos of Zea mays L. from suspension cultures. Journal of Plant Physiology 142: 597-604.
• Friml J. 2003. Auxin transport - shaping the plant. Current Opinion in Plant Biology 6: 7-12.
• Hofmann N, Nelson RL, and Korban SS. 2004. Influence of media components and pH on somatic embryo induction in three genotypes of soybean. Plant Cell, Tissue and Organ Culture 77: 157-163.
• Hu Q, Andersen SB, and Hansen LN. 1999. Plant regeneration capacity of mesophyll protoplasts from Brassica napus and related species. Plant Cell, Tissue and Organ Culture 59: 189-196.
• Jimenez VM, and Bangerth F. 2001. Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot. Physiologia Plantarum 111: 389-395.
• Jourdan PS, and Earle ED. 1989. Genotypic variability in the frequency of plant regeneration from leaf protoplasts of four Brassica ssp. and Raphanus sativus. Journal of the American Society for Horticultural Science 114: 343-349.
• Khan MR, Rashid H, and Quraishi A. 2002. Effects of various growth regulators on callus formation and regeneration in Brassica napus cv. Oscar. Pakistan Journal of Biological Sciences 5: 693-695.
• Klimaszewska K, and Keller WA. 1985. High frequency plant regeneration from thin cell layer explants of Brassica napus. Plant Cell, Tissue and Organ Culture 4: 183-197.
• Leyser O. 2001. Auxin signalling: the beginning, the middle and the end. Current Opinion in Plant Biology 4: 382-386.
• Lu MC. 2004. High frequency plant regeneration from callus culture of Pleione formosana Hayata. Plant Cell, Tissue and Organ Culture 78: 93-96.
• Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
• Pellegrineschi A, Brito RM, McLean S, and Hoisington D. 2004. Effect of 2,4-dichlorophenoxyacetic acid and NaCl on the establishment of callus and plant regeneration in durum and bread wheat. Plant Cell, Tissue and Organ Culture 77: 245-250.
• Petrášek J, Elčkner M, Morris DA, and Zažímalová E. 2002. Auxin efflux carrier activity and auxin accumulation regulate cell division and polarity in tobacco cells. Planta 216: 302-308.
• Rogozińska JH, and Drozdowska L. 1980. Organogenesis and plant formation from cotyledon and callus culture of rape. Acta Societatis Botanicorum Poloniae 49: 5-20.
• Rück A, Palme K, Venis MA, Napier RM, and Felle HH. 1993. Patch-clamp analysis establishes a role for an auxin binding protein in the auxin stimulation of plasma membrane current in Zea mays protoplasts. The Plant Journal 4: 41-46.
• Sairam RV, Franklin G, Hassel R, Smith B, Meeker K, Kashikar N, Parani M, Abed DA, Ismail S, Berry K, and Goldman SL. 2003. A study on an effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell, Tissue and Organ Culture 75: 79-85.
• Ullah I, Rashid H, and Khan MR. 2004. Establishment of tissue culture protocol in Brassica (Brassica napus L.). Pakistan Journal of Biological Sciences 7: 277-278.
• Vikrant, and Rashid A. 2003. Somatic embryogenesis or shoot formation following high 2,4-D pulse - treatment of mature embryos of Paspalum scrobiculatum. Biologia Plantarum 46: 297-300.
• Wang CT, and Wei ZM. 2004. Embryogenesis and regeneration of green plantlets from wheat (Triticum aestivum) leaf base. Plant Cell, Tissue and Organ Culture 77: 149-156.
• Zhang L, Rybczynski JJ, Langerberg WG, Mitra A, and French R. 2000. An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration. Plant Cell Reports 19: 241-250.
• Zheng MY, and Konzak CF. 1999. Effect of 2,4-dichlorophenoxyacetic acid on callus induction and plant regeneration in anther culture of wheat (Triticum aestivum L.). Plant Cell Reports 19: 69-73.