Expression of the plum pox coat protein gene in transgenic Nicotiana tabacum plants

• Autorzy: Wypijewski K. J. , Musial W.G. , Misztal L. , Pater B. , Malinowski T. , Augustyniak J.
• Języki publikacji: EN

Abstrakty

EN

Plant expression vector pBI 121 containing the gene encoding coat protein of Plum Pox Virus of the Skierniewice isolate (CP PPV-S) was prepared (clone pCMl). The construct was used for transformation of Nicotiana tabacum plants using an Agrobacterium tumefaciens based system. About 82% of kanamycin resistant plant lines contained a transgene (the sequence of CP PPV-S) but only 81% of them actively expressed the PPV-S coat protein gene as measured by RT-PCR.

Słowa kluczowe

EN

plant transformation; plum pox virus; Agrobacterium tumefaciens; transgenic plant; kanamycin resistance; Nicotiana tabacum; coat protein gene

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1995
• Tom: 42
• Numer: 1
• Opis fizyczny: p.97-101,fig.

Twórcy

autor

Wypijewski K. J.

• A.Mickiewicz University, Miedzychodzka 5, 60-371 Poznan, Poland

autor

Musial W.G.

autor

Misztal L.

autor

Pater B.

autor

Malinowski T.

autor

Augustyniak J.

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