Micropropagation of six Prunus mume cultivars through axillary shoot proliferation, and ISSR analysis of cloned plants

• Autorzy: Ning G G , Fan X.L. , Huang W.J. , Bao M.Z. , Zhang J.B.
• Języki publikacji: EN

Abstrakty

EN

Prunus mume is one of the most popular landscape plants In China and Japan. A successful in vitro propagation system for six cultivars of Prunus mume has been developed by in vitro culture of nodal segments from seedling and mature plants. High multiplication rates (from 2.5 to 5.5) were achieved using modified MS media and WPM basic media supplemented with TDZ, BA, IBA, 2,4-D or NAA at concentrations adjusted for each cultivar. All the studied cultivars could be proliferated efficiently on WPM media supplemented with 2.2 µM TDZ, 2.2 µM BA and 2.5 µM IBA. Shoots were rooted on agar-gelled 1/2 MS or WPM basic media containing 2.5 or 5.0 µM IBA, and plantlets were transferred to pots after they had grown more than 3 roots and at least one root was more than 10 mm long. The effects of TDZ, media composition and different genotypes on shoot multiplication and growth were studied in detail. The genetic fidelity of the micropropagated plants from the ’Xuemei’ cultivar was examined using PCR-ISSR markers, and the results demonstrated complete genetic stability in the cloned plants.

Słowa kluczowe

EN

micropropagation; Prunus mume; plant cultivar; shoot proliferation; clonal plant; axillary shoot; Japanese apricot; Chinese plum zob.Japanese apricot; ISSR technique; tissue culture; genetic stability

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2007
• Tom: 49
• Numer: 1
• Opis fizyczny: p.25-31,fig.,ref.

Twórcy

autor

Ning G G

• Huazhong Agricultural University, Wuhan 430070, P.R.China

autor

Fan X.L.

autor

Huang W.J.

autor

Bao M.Z.

autor

Zhang J.B.

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