Polymorphisms at loci of leptin [LEP], Pit1 and STAT5A and their association with growth, feed conversion and carcass quality in Black-and-White bulls

• Autorzy: Oprzadek J , Flisikowski K. , Zwierzchowski L. , Dymnicki E.
• Języki publikacji: EN

Abstrakty

EN

The association was studied between the polymorphism at leptin (LEP), Pit1, and STAT5A loci and meat production traits in 145 Black-and-White growing/fattening bulls. Genotypes of LEP, Pit1 and STAT5A were determined with the PCR-RFLP technique. Over the 8th month of age the 28-day performance test was introduced to assess growth rate and feed conversion during which the fullconcentrate diet was offered ad libitum. At the age of 15 months the bulls were slaughtered, and their carcasses cut and dissected into lean, fat and bone.The allele frequencies were 0.85, 0.07, and 0.08 for A, B and C LEP variants, 0.25 and 0.75 for A and B Pit1 variants, and 0.90 and 0.10 for C and T STAT5A variants, respectively. Polymorphism of lepton significantly affected some carcass traits, and among them the weight of carcass-side that was highest in the AA homozygotes. The effect of Pit1 genotype was observed on carcass dimensions only. The AA homozygotes had higher chest circumference, chest depth, and circumference of round, but BB homozygotes had a higher round width. CT genotype of the STAT5A-encoding gene significantly affected four out of 36 carcass traits measured and was related to higher weight of bone of best + fore ribs (4.2 vs 3.8 kg) and of sirloin (1.6 vs 1.3 kg) as well as to oblique carcass length (140.5 vs 138.5 cm). The CC STAT5A genotype was associated with significantly higher live weight gain from 8 to 15 months (1.04 vs 0,97 kg daily).

PL

Celem pracy było określenie genetycznej zmienności w obrębie loci genów LEP, Pit1 i STAT5A,a także zbadanie wpływu ich alleli na tempo wzrostu, spożycie i wykorzystanie paszy oraz użytkowość rzeźną bydła czarno-białego. Materiał stanowiło 145 buhajków rasy cb opasanych do 15 miesiąca życia, a następnie ubijanych. Między 7 a 8 miesiącem życia buhajków przeprowadzono 28-dniowy test dla określenia pobierania i wykorzystania paszy, podczas którego zwierzęta żywiono wyłącznie paszą treściwą. Genotypy wymienionych czynników określono techniką PCR-RFLP. Analizie poddano 36 cech tuszy określonych w wyniku ich pomiarów liniowych, rozbioru i dysekcji na mięso, tłuszcz i kości. W obrębie genotypów leptyny zwierzęta o genotypie AA miały większą masę dysekowanej półtuszy. Osobniki AA pobierały więcej suchej masy, białka ogólnego oraz energii netto wyrażonej w jednostkach produkcji żywca (UFV) niż osobniki AB i AC. Wystąpiły różnice między zwierzętami o różnym genotypie Pit1 – homozygoty BB pobierały mniej paszy i jej składników niż heterozygoty AB. Nie stwierdzono zależności między Pit1 a masą mięsa, tłuszczu i kości w tuszy. W obrębie genotypów STAT5A buhajki CC miały mniejszą masę kości w antrykocie, rozbratelu i rozbefie, a ich tusze były krótsze niż buhajków CT. Jednocześnie osobniki CC wykazały istotnie wyższe przyrosty masy ciała.

Słowa kluczowe

EN

Black-and-White breed; cattle; bull; young animal; polymorphism; leptin; gene; carcass quality; slaughter performance; meat production; fattening; growth rate; feed conversion

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2003
• Tom: 21
• Numer: 3
• Opis fizyczny: p.135-145

Twórcy

autor

Oprzadek J

• Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland

autor

Flisikowski K.

autor

Zwierzchowski L.

autor

Dymnicki E.

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