Molecular characterization and symbiotic importance of prsD gene of Rhizobium leguminosarum bv.trifolii TA1

• Autorzy: Mazur A. , Wielbo J. , Krol J. , Kopcinska J. , Lotocka B. , Golinowski W. , Skorupska A.
• Języki publikacji: EN

Abstrakty

EN

The prsD, prsE and orf3 genes of Rhizobium leguminosarum bv. trifolii strain TA1 encode the proteins which are significantly related to the family of bacterial ABC transporters type I secretion systems. The prsD:Kmr mutant of strain TA1 induced non-nitrogen-fixing nodules on Trifolium pratense. Microscopic analysis of the nodules induced by prsD mutant did not reveal major abberations in the bacteroid appearance. The exopolysaccharide of prsD mutant was produced in increased amount and its level of polymerization was changed. SDS/PAGE of the proteins from the culture supernatants showed a lack of the 47-kDa protein in the culture of prsD mutant. Thus, PrsD may play a role in the export of this protein.

Słowa kluczowe

EN

Rhizobium leguminosarum bv.trifolii; nodule; Trifolium pratense; orf3 gene; prsD gene; bacterial mutant; exopolysaccharide; gene; prsD mutant; prssE gene; protein; rhizobial exopolysaccharide; mutation

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1998
• Tom: 45
• Numer: 4
• Opis fizyczny: p.1067-1073,fig.

Twórcy

autor

Mazur A.

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Wielbo J.

autor

Krol J.

autor

Kopcinska J.

autor

Lotocka B.

autor

Golinowski W.

autor

Skorupska A.

Bibliografia

• Becker, A., Schmidt, M., Jäger, W. & Piihler, A. (1995) Gene 162, 37-39.
• Bialek, U., Skorupska, A., Yang, W.-C., Bisseling, T. & Van Lammeren, A.A.M. (1995) Planta 197, 184-192.
• Brown, C.M. & Dilworth, M.J. (1975) J. Gen. Mi­crobiol 122, 61-67.
• Chen, H., Batley, M., Redmond, J. & Rolfe, B. (1985) J. Plant Physiol 120, 331-349.
• Dart, P.J. & Mercer, F.V. (1963) Arch. Mikrobiol 47, 1-18.
• Derylo, M., Skorupska, A., Bednara, J. & Lorkie- wicz, Z. (1986) Physiol Plant. 66, 699-704.
• Ditta, G., Stanfield, S., Corbin, D. & Helinski, D. R. (1980) Proc. Natl Acad, Sei. U.S.A. 77, 7347-7351.
• Duong, F., Lazdunski. A., Cami. B. & Murgier. M. (1992) Gene 121, 47-54.
• Finan, T.M., Hirsch, A.M., Leigh, J.A., Johansen, E. , Kuldan, G.A., Deegan, S., Walker, G.C. & Singer, E.R. (1985) Cell 40, 869-877.
• Finnie, C. Hartley, N.M., Findlay, K.C. & Downie, J.A. (1997) Mol Microbiol 25, 135-146./
• Finnie, C., Zorrequieta, A., Hartley, N.M. & Downie, A. (1998) J. Bacterial. 180, 1691- 1699.
• Król, J. & Skorupska, A. (1997) Microbiology 143, 1389-1394.
• Leigh, J.A. & Walker, G.C. (1994) Trends Genet 10, 63-67.
• Letoffś. S., Depelaire, P. & Wandersman, C. (1990) EMBO J. 9, 1375-1382.
• Lever, M. (1972) Analyt. Biochem. 47, 273-279.
• Loewus, F.A. (1952) Anal. Chem. 24, 219.
• Łotocka, B., Kopcińska, J. & Golinowski, W. (1997) Acta Soc. Bot. Polon. 66. 273-292.
• Mateos, P.F., Jimenez-Zurdo, J.I., Chen, J., Squartini, A.S., Haack, S.K., Martinez- Molina, E., HubbeU, D.H. & Dazzo, F.B. (1992) Appi Environ. Microbiol 58, 1816-1822.
• Miller, J.H. (1972) Experiments in molecular genet­ics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
• Niehaus, K., Kapp, D. & Pühler, A. (1993) Planta 190, 415-425.
• Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, N.Y.
• Simon, R., Priefer, U. & Pühler, A. (1983) Bio/ Technology 1, 784-791.
• Skorupska, A., Bialek, U., Urbanik-Sypniewska, T. & Van Lammeren, A. (1995) J. Plant Physiol 147, 93-100.
• Spaink, H.P., Okker, R.J.H., Wijffelman, C.A., Pees, E. & Lugtenberg, B.JJ. (1987) Plant Mol Biol 9, 27-39.
• Vasse, J., de Billy, F., Camut, S. & Truchet, G. (1990) J. Bacteriol. 172, 4295-4306.
• Vincent, M. (1970) A Mannual for the Practical Study of Root-Nodule Bacteria. International Biological Programme. Handbook No. 15. Blackwell Sei. Publ., Oxford, Edinburgh
• York, G.M. & Walker, G.C. (1997) Mol Microbiol 25,117-134.