Immunolocalization of calreticulin in protoplasts and somatic embryos of Daucus carota L. grown in suspension culture

• Autorzy: Libik M. , Przywara L.
• Języki publikacji: EN

Abstrakty

EN

Ca2+ acts as a second messenger which controls a wide variety of cellular functions. Ca2+ homeostasis is accomplished by a complex of mechanisms including pumps, ion channels and calcium buffers within storage compartments. Calreticulin is the low-affinity, high-capacity Ca2+-binding protein of most eucaryotic cells. The distribution of calreticulin was studied in isolated protoplasts and during different stages of somatic embryogenesis in carrot (Daucus carota L. cv. St Valery Lunga Rossa) suspension culture. Immunofluorescence staining of protoplasts showed the presence of calreticulin in the cytoplasm. No calreticulin was observed within vacuoles. During somatic embryogenesis calreticulin was distributed mainly in the protoderm of developing embryos in different stages.

Słowa kluczowe

EN

immunofluorescence; plant physiology; Daucus carota; eukaryotic cell; protoplast; somatic embryogenesis; calreticulin

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2000
• Tom: 42
• Numer: 2
• Opis fizyczny: p.87-92,fig.

Twórcy

autor

Libik M.

• Polish Academy of Sciences, Slawkowska 17, 31-017 Krakow, Poland

autor

Przywara L.

Bibliografia

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