Wplyw kadmu na aktywnosc enzymow antyoksydacyjnych w izolowanych hepatocytach szczura

• Autorzy: Czeczot H , Scibior-Bentkowska D. , Skrzycki M. , Podsiad M. , Karlik W. , Bakala A. , Grono D. , Wiechetek M.

Warianty tytułu

EN

Effect of cadmium on the activity of antioxidant enzymes in isolated rat hepatocytes

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was to evaluate the effect of cadmium on the antioxidant enzyme activity in rat hepatocytes. The experiments were conducted on isolated rat hepatocytes, which were incubated for two hours in modified Williams’ E medium (MWE) with 25, 50, and 200 µM cadmium chloride (CdCl₂). Hepatocytes incubated in MWE medium without cadmium chloride were used as a control. The activity of antioxidant enzymes - superoxide dismutases (SOD1 and SOD2), catalase (CAT), total glutathione peroxidase (tot. GSHPx), selenium-dependent glutathione peroxidase (Se-GSHPx), glutathione S-transferase (GST) and glutathione reductase (GSHR) - and the values of the studied oxidative stress markers - the concentration of tiobarbituric-acid-reacting substances (TBARS) and reduced glutathione (GSH) - indicate that cadmium induces oxidative stress in rat hepatocytes, which impairs antioxidative mechanisms.

Słowa kluczowe

PL

metale ciezkie; kadm; toksycznosc; hepatocyty; izolowane hepatocyty szczura; badania in vitro; stres oksydacyjny; enzymy antyoksydacyjne; aktywnosc enzymatyczna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2009
• Tom: 65
• Numer: 01
• Opis fizyczny: s.55-60,rys.,tab.,bibliogr.

Twórcy

autor

Czeczot H

• Uniwersytet Medyczny w Warszawie, ul.Banacha 1, 02-097 Warszawa

autor

Scibior-Bentkowska D.

autor

Skrzycki M.

autor

Podsiad M.

autor

Karlik W.

autor

Bakala A.

autor

Grono D.

autor

Wiechetek M.

Bibliografia

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