Identification of actin from hepatoma Morris 5123 cells

• Autorzy: Nowak D. , Kochman A. , Malicka-Blaszkiewicz M.
• Języki publikacji: EN

Abstrakty

EN

The hepatoma Morris 5123 tumor growth is accompanied by changes in actin content and polymerization (Malicka-Błaszkiewicz et al. (1995) Mat. Med. Pol., 27, 115-118; Nowak et al. (1995) J. Exp. Cancer Res. 14, 37-40). Presently actin isoforms from cytosol and cytoskeleton fractions were separated by SDS/PAGE and identified with antibodies directed against different actin isoforms. Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit α skeletal muscle actin (Mr 43000) and isoelectric point equal to 5.3. It interacts only with monoclonal anti β actin isoform antibodies, posing the question of differential affinity of actin isoforms to DNase I.

Słowa kluczowe

EN

affinity chromatography; cytoskeleton fraction; cell; polymerization; actin; cell pathology; Morris hepatoma 5123; tumour growth; cytosol fraction; actin isoform; identification

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 4
• Opis fizyczny: p.949-959,fig.

Twórcy

autor

Nowak D.

• University of Wroclaw, S.Przybyszewskiego 63, 51-148 Wroclaw, Poland

autor

Kochman A.

autor

Malicka-Blaszkiewicz M.

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