Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis [2-DE]
Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14®) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).
Aoyagi Y., E.E. Adderson, C.E. Rubens, J.F. Bohnsack, J.G. Min, M. Matsushita, T. Fujita, Y. Okuwaki and S. Takahashi. 2008. L-ficolin/mannose-binding lectin-associated serine protease complexes bind to group B streptococci primarily through N-Acetylneuraminic acid of capsular polysaccharide and activate the complement pathway. Infect. Immun. K76: 179-188.
Badger J.L. and K.S. Kim. 1998. Environmental growth conditions influence the ability of Escherichia coli K1 to invade brain microvascular endothelial cells and confer serum resistance. Infect. Immun. 66: 5692-5697.
Bugla G., A. Korzeniowska-Kowal, A. Gamian and W. Doroszkiewicz. 2004. Bactericidal activity of serum against Salmonella O48 serovars. Int. J. Antimicroh. Agents 24: 604.
Bugla-Płoskońska G. and W. Doroszkiewicz. 2006. Bactericidal activity of normal bovine serum (NSB) directed against some Enterohacteriaceae w(th sialic acid-containing lipopolysaccharides (LPS) as a component of cell wall. Pol. J. Microbiol. 55: 169-174.
Connolly J.P., D. Comerci, T.G. Alefantis, A. Walz, M. Quan, R. Chafin, P. Grewal, C.V. Mujer, R.A. Ugalde and V.G. DelVecchio. 2006. Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development. Proteomics 6: 3767-3780.
Cullen P.A., S.J. Cordwell, D.M. Bulach, D.A. Haake and B. Adler. 2002. Global analysis of outer membrane proteins from Leptospira interrogans serovar Lai. Infect. Immun. 70: 2311-2318.
Fountoulakis M. 2005. Analysis of Membrane Proteins by Two-Dimensional Gels. The Proteomics Protocols Handbook, ed. John M. Walker. Humana Press: 133-144.
Futoma B., G. Bugla-Płoskońska and W. Doroszkiewicz. 2005. Bactericidal complement activity against Salmonella enterica strains. Pol. J. Emir. Stud. 14 suppl. II: 101-104.
Gamian A., L. Kenne, M. Mieszała, J. Urlich and J. Defaye. 1994. Structure of the Escherichia coli O24 and O56 O-specific sialic acid-containing polysaccharides and linkage of these structures to the core region in lipopolysaccharides. Eur. J. Biochem. 225: 1211-1220.
Hamid N. and S.K. Jain. 2008. Characterization of an outer membrane protein of Salmonella enterica serovar typhimurium that confers protection against typhoid. Clin. Vaccine Immunol. 15: 1461-1471.
Henningsen R., B.L. Gale, K.M. Straub and D.C. DeNagel. 2002. Application of zwitterionic detergents to the solubilization of integral membrane proteins for two-dimensional gel electrophoresis and mass spectrometry. Proteomics 2: 1479-1488.
Jessouroun E., I.F. Silveira, A.P. Larangeira, S. Pereira, S.A. Fernandes, L. Rabinovitch, C.E. Frasch, H.C. Castro-Faria-Neto and P.T. Bozza. 2004. Outer membrane vesicles (OMVs) and detoxified lipooligosaccharide (dLOS) obtained from Brazilian prevalent N. meningitidis serogroup B strains protect mice against homologous and heterologous meningococcal infection and septic shock. Vaccine 22: 2617-2625.
Kawai K., Y. Liu, K. Ohnishi and S. Oshima. 2004. A conserved 37 kDa outer membrane protein of Edwardsiella tarda is an effective vaccine candidate. Vaccine 22: 3411-3418.
Kroll H.P., S. Bhakdi and P.W. Taylor. 1983. Membrane changes induced by exposure of Escherichia coli to human serum. Infect. Immun. 12: 1055-1066.
Laemmli U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 15: 680-685.
Luche S., V. Santoni and T. Rabilloud. 2003. Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3: 249-253.
Mielnik G., A. Gamian and W. Doroszkiewicz. 2001. Bactericidal activity of normal cord serum (NCS) against Gram-negative rods with sialic acid-containing lipopolysacchrides (LPS). FEMS Immunol. Med. Microbiol. 31: 169-173.
Molloy M.P., B.R. Herbert, M.B. Slade, T. Rabilloud, A.S. Nouwens, K.L. Williams and A.A. Gooley. 2000. Proteomic analysis of the Escherichia coli outer membrane. Eur. J. Biochem. 267: 2871-2881.
Murphy T.F. and L.C. Bartos. 1989. Surface-exposed and anti-genically conserved determinants of outer membrane proteins of Branhamella catarrhalis. Infect. Immun. 57: 2938-2941.
O'Farrell P. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biolog. Chem. 250: 4007-4021.
Orskov I., F. Orskov, B. Jann and K. Jann. 1977. Serology, chemistry and genetics of O and K antigens of Escherichia coli. Bacteriol. Rev. 41: 667-710.
Peng X., X. Ye and S. Wang. 2004. Identification of novel immunogenic proteins of Shigella flexneri 2a by proteomic methodologies. Vaccine 22: 2750-2756.
PIERCE Instructions. Pierce®BCA Protein Assay kit. Thermo Scientific, Pierce Biotechnology.
Rockwood D., M.T. Wilson and V.M. Darley-Usmar. 1987. Isolation and characteristic of intact mitochondria, pp. 1-16. In: V.M. Darley-Usmar, D. Rickwood, M.T. Wilson (eds). Mitochondria: a practical approach. IRL Press, Oxford.
Shaw M.M. and B.M. Riederer. 2006. Sample preparation for two-dimensional gel electrophoresis. Current Advancements in the Methodology. G.I.T. Laboratory Journal 6: 302-303.
Smith P.K., R.I. Krohn, G.T. Hermanson, A.K. Mallia, F.H. Gartner, M.D. Provenzano, E.K. Fujimoto, N.M. Goeke, B.J. Olson and D.C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150: 76-85.
Sorichter S., U. Baumann, A. Baumgart, S. Walterspacher and B.U. Specht. 2009. Immune responses in the airways by nasal vaccination with systemic boosting against Pseudomonas aeruginosa in chronic lung disease. Vaccine 27: 2755-2759.
Srirama M.B. 2001. A guide to the properties and uses of detergents in biology and biochemistry. Calbiochem Manual. Taylor P.W. and R. Parton. 1976. A protein factor associated with serum resistance in Escherichia coli. J. Med. Microbiol. 10: 225-232.
Vimr E. and C. Lichtensteiger. 2002. To sialylate, or not to sialylate: that is the question. Trends Microbiol. 10: 254-257.
Witkowska D., E. Masłowska, M. Staniszewska, B. Szostko, A. Jankowski and A. Gamian. 2006. Enterobacterial 38-kDa outer membrane protein is an age-dependent molecular marker of inna te immunity and immunoglobulin deficiency as results from its reactivity with IgG and IgA antibody. FEMS Immunol. Med. Microbiol. 48: 205-214.