Use of zwitterionic type of detergent in isolation of Escherichia coli O56 outer membrane proteins improves their two-dimensional electrophoresis [2-DE]

• Autorzy: Bugla-Ploskonska G , Futoma-Koloch B. , Skwara A. , Doroszkiewicz W.
• Języki publikacji: EN

Abstrakty

EN

Escherichia coli O56 were originally isolated from infected humans. Here it is reported that using the zwitterionic detergent (Zwittergent Z 3-14®) to isolate outer membrane proteins (OMPs) from Escherichia coli O56 is suitable for their separation by two-dimensional electrophoresis (2-DE) using pH 3-10 immobilized pH gradient IPG strips (BIO-RAD).

Słowa kluczowe

EN

electrophoresis; bacteria; Escherichia coli; zwitterionic detergent; proteomics; membrane protein; two-dimensional electrophoresis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2009
• Tom: 58
• Numer: 3
• Opis fizyczny: p.205-209,fig.,ref.

Twórcy

autor

Bugla-Ploskonska G

• University of Wroclaw, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland

autor

Futoma-Koloch B.

autor

Skwara A.

autor

Doroszkiewicz W.

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