Capillary zone electrophoresis [CZE] of flaxseed phenolic constituents - a short report

• Autorzy: Rybarczyk A. , Pegg R.B. , Amarowicz R.
• Języki publikacji: EN

Abstrakty

EN

Phenolic compounds were extracted from flaxseed using 80% (v/v) methanol. The resultant crude extract was hydrolysed under basic conditions. Secoisolariciresinol diglucoside (SDG) was separated from the prepared extract using semi-preparative HPLC. Secoisolariciresinol (SECO) was liberated from SDG by acid hydrolysis. The chemical structures of SDG and SECO were confirmed by ESI-MS. Capillary zone electrophoresis (CZE) was employed for the separation of phenolics from the crude extract, the extract resulting after base hydrolysis, and the two lignans. CZE was found to offer a quicker analysis of SDG with higher separation efficiency than with a conventional HPLC methodology. The retention time for SDG was noted at 5.33 min. The migration times of SDG and SECO were close together; a base line separation of both lignans was not achieved. The electropherogram of the crude flaxseed extract showed only one broad peak with a retention time of 6.02 min.

Słowa kluczowe

EN

fatty acid; flax seed; capillary zone electrophoresis; Linum usitatissimum; phenolic compound; omega-3 fatty acid; lignan; alpha-linolenic acid

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2008
• Tom: 58
• Numer: 2
• Opis fizyczny: p.263-267,fig.,ref.

Twórcy

autor

Rybarczyk A.

• Polish Academy of Sciences, Tuwima 10, P.O.Box 55, 10-747 Olsztyn, Poland

autor

Pegg R.B.

autor

Amarowicz R.

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